TY - JOUR
T1 - Genetic architecture of limit dextrinase inhibitor (LDI) activity in Tibetan wild barley
AU - Huang, Yuqing
AU - Cai, Shengguan
AU - Ye, Lingzhen
AU - Han, Yong
AU - Wu, Dezhi
AU - Dai, Fei
AU - Li, Chengdao
AU - Zhang, Guoping
N1 - Funding Information:
This research was supported by National Natural Science Foundation of China (Nos. 31330055, 31271656, 31129005), Zhejiang Provincial Natural Science Foundation of China (No. Z3110054) and China Agriculture Research System. We thank Mr Xianyin Zhang and Ms. Mei Li, the technicians of 985-Institute of Agrobiology for providing convenience in using the experimental equipments.
PY - 2014/5/1
Y1 - 2014/5/1
N2 - Background: Limit dextrinase inhibitor (LDI) inhibits starch degradation in barley grains during malting because it binds with limit dextrinase (LD). There is a wide genetic variation in LDI synthesis and inactivation during barley grain development and germination. However, the genetic control of LDI activity remains little understood.Results: In this study, association analysis was performed on 162 Tibetan wild accessions by using LDI activity, 835 Diversity Arrays Technology (DArT) markers and single nucleotide polymorphisms (SNPs) of the gene HvLDI encoding LDI. Two DArT markers, bpb-8347, bpb-0068, and 31 SNPs of HvLDI were significantly associated with LDI activity, explaining 10.0%, 6.6% and 13.4% of phenotypic variation, respectively. Bpb-8347 is located on chromosome 6H, near the locus of HvLDI, and bpb-0068 is located on 3H.Conclusions: The current results confirmed the locus of the gene controlling LDI activity and identified a new DArT markers associated with LDI activity. The SNPs associated with LDI activity may provide a new insight into the genetic variation of LDI activity in barley grains.
AB - Background: Limit dextrinase inhibitor (LDI) inhibits starch degradation in barley grains during malting because it binds with limit dextrinase (LD). There is a wide genetic variation in LDI synthesis and inactivation during barley grain development and germination. However, the genetic control of LDI activity remains little understood.Results: In this study, association analysis was performed on 162 Tibetan wild accessions by using LDI activity, 835 Diversity Arrays Technology (DArT) markers and single nucleotide polymorphisms (SNPs) of the gene HvLDI encoding LDI. Two DArT markers, bpb-8347, bpb-0068, and 31 SNPs of HvLDI were significantly associated with LDI activity, explaining 10.0%, 6.6% and 13.4% of phenotypic variation, respectively. Bpb-8347 is located on chromosome 6H, near the locus of HvLDI, and bpb-0068 is located on 3H.Conclusions: The current results confirmed the locus of the gene controlling LDI activity and identified a new DArT markers associated with LDI activity. The SNPs associated with LDI activity may provide a new insight into the genetic variation of LDI activity in barley grains.
KW - Genome-wide association study (GWAS)
KW - Limit dextrinase inhibitor (LDI)
KW - Single nucleotide polymorphism (SNP)
KW - Tibetan wild barley
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U2 - 10.1186/1471-2229-14-117
DO - 10.1186/1471-2229-14-117
M3 - Article
C2 - 24885294
AN - SCOPUS:84901694359
SN - 1471-2229
VL - 14
JO - BMC Plant Biology
JF - BMC Plant Biology
IS - 1
M1 - 117
ER -