Genetic architecture of limit dextrinase inhibitor (LDI) activity in Tibetan wild barley

Yuqing Huang, Shengguan Cai, Lingzhen Ye, Yong Han, Dezhi Wu, Fei Dai, Chengdao Li, Guoping Zhang

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)


Background: Limit dextrinase inhibitor (LDI) inhibits starch degradation in barley grains during malting because it binds with limit dextrinase (LD). There is a wide genetic variation in LDI synthesis and inactivation during barley grain development and germination. However, the genetic control of LDI activity remains little understood.Results: In this study, association analysis was performed on 162 Tibetan wild accessions by using LDI activity, 835 Diversity Arrays Technology (DArT) markers and single nucleotide polymorphisms (SNPs) of the gene HvLDI encoding LDI. Two DArT markers, bpb-8347, bpb-0068, and 31 SNPs of HvLDI were significantly associated with LDI activity, explaining 10.0%, 6.6% and 13.4% of phenotypic variation, respectively. Bpb-8347 is located on chromosome 6H, near the locus of HvLDI, and bpb-0068 is located on 3H.Conclusions: The current results confirmed the locus of the gene controlling LDI activity and identified a new DArT markers associated with LDI activity. The SNPs associated with LDI activity may provide a new insight into the genetic variation of LDI activity in barley grains.

Original languageEnglish
Article number117
JournalBMC Plant Biology
Issue number1
Publication statusPublished - May 1 2014
Externally publishedYes


  • Genome-wide association study (GWAS)
  • Limit dextrinase inhibitor (LDI)
  • Single nucleotide polymorphism (SNP)
  • Tibetan wild barley

ASJC Scopus subject areas

  • Plant Science


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