TY - JOUR
T1 - Genetic characterization of human respiratory syncytial virus detected in hospitalized children in the Philippines from 2008 to 2012
AU - Ohno, Ayumu
AU - Suzuki, Akira
AU - Lupisan, Socorro
AU - Galang, Hazel
AU - Sombrero, Lydia
AU - Aniceto, Rapunzel
AU - Okamoto, Michiko
AU - Saito, Mariko
AU - Fuji, Naoko
AU - Otomaru, Hirono
AU - Roy, Chandra Nath
AU - Yamamoto, Dai
AU - Tamaki, Raita
AU - Olveda, Remigio
AU - Oshitani, Hitoshi
N1 - Funding Information:
This work was supported by a grant-in-aid from the Japan Initiative for Global Research Network on Infectious Diseases (J-GRID) , the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan and Science and Technology Research Partnership for Sustainable Development (SATREPS) from Japan Science and Technology Agency (JST) and Japan International Cooperation Agency (JICA) .
PY - 2013/5
Y1 - 2013/5
N2 - Background: Human respiratory syncytial virus (HRSV) is the leading cause of acute lower respiratory tract infection in infants and young children. However, molecular characteristic of HRSV is still unknown in the Philippines. Objective: To describe the molecular epidemiology of circulating HRSV detected in the Philippines. Study design: From May 2008 to April 2012, nasopharyngeal swabs were collected from infants and children aged between 7 days and 14 years who were hospitalized with severe pneumonia. HRSV was detected by nested PCR targeting M2 gene, and C-terminus of the G gene was sequenced for phylogenetic analysis. Result: Out of total 2150 samples, 19.3% (n= 415) were positive for HRSV, and 65.0% of them (n= 270) were identified as HRSV-A and 35.0% (n= 145) as HRSV-B. There were two major HRSV outbreaks: between June 2008 and February 2009, and between June and March 2012. Majority of HRSV strains detected during the former outbreak were HRSV-A (97.5%, 203/208) whereas during the later outbreak, both HRSV-A (54/158, 34.2%) and HRSV-B (104/158, 65.8%) were detected. All HRSV-A strains were classified as genotype NA1 and all HRSV-B as genotype BA, which had 60-nucleotide duplication in secondary hypervariable region of the G gene. Among HRSV-B positive samples, there were 2 distinct clusters with unique amino acid changes and low homology in compared to other strains in BA, suggesting emergence of new variant of HRSV-B. Conclusion: The study provides an overview of the genetic variation in circulating HRSV viruses in the Philippines along with identification of possibly a novel variant of HRSV-B.
AB - Background: Human respiratory syncytial virus (HRSV) is the leading cause of acute lower respiratory tract infection in infants and young children. However, molecular characteristic of HRSV is still unknown in the Philippines. Objective: To describe the molecular epidemiology of circulating HRSV detected in the Philippines. Study design: From May 2008 to April 2012, nasopharyngeal swabs were collected from infants and children aged between 7 days and 14 years who were hospitalized with severe pneumonia. HRSV was detected by nested PCR targeting M2 gene, and C-terminus of the G gene was sequenced for phylogenetic analysis. Result: Out of total 2150 samples, 19.3% (n= 415) were positive for HRSV, and 65.0% of them (n= 270) were identified as HRSV-A and 35.0% (n= 145) as HRSV-B. There were two major HRSV outbreaks: between June 2008 and February 2009, and between June and March 2012. Majority of HRSV strains detected during the former outbreak were HRSV-A (97.5%, 203/208) whereas during the later outbreak, both HRSV-A (54/158, 34.2%) and HRSV-B (104/158, 65.8%) were detected. All HRSV-A strains were classified as genotype NA1 and all HRSV-B as genotype BA, which had 60-nucleotide duplication in secondary hypervariable region of the G gene. Among HRSV-B positive samples, there were 2 distinct clusters with unique amino acid changes and low homology in compared to other strains in BA, suggesting emergence of new variant of HRSV-B. Conclusion: The study provides an overview of the genetic variation in circulating HRSV viruses in the Philippines along with identification of possibly a novel variant of HRSV-B.
KW - Childhood pneumonia
KW - Genotype
KW - Respiratory syncytial virus
KW - The Philippines
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U2 - 10.1016/j.jcv.2013.01.001
DO - 10.1016/j.jcv.2013.01.001
M3 - Article
C2 - 23357644
AN - SCOPUS:84875805116
SN - 1386-6532
VL - 57
SP - 59
EP - 65
JO - Journal of Clinical Virology
JF - Journal of Clinical Virology
IS - 1
ER -
