TY - JOUR
T1 - Genetic characterization of Pseudomonas aeruginosa bacteriophage KPP10
AU - Uchiyama, Jumpei
AU - Rashel, Mohammad
AU - Takemura, Iyo
AU - Kato, Shin ichiro
AU - Ujihara, Takako
AU - Muraoka, Asako
AU - Matsuzaki, Shigenobu
AU - Daibata, Masanori
N1 - Funding Information:
We thank Mr. Shingo Sakurai for his technical support, and thank the Science Research Center, Kochi, Japan, for its experimental support. This study was funded by a Grant-in-Aid for Research Activity Startup (no. 22890129), Kochi System Glycobiology Center, Kochi, Japan, and the Center of Biomembrane Functions Controlling Biological Systems, Kochi, Japan.
PY - 2012/4
Y1 - 2012/4
N2 - Bacteriophage (phage) KPP10 has been used in experimental phage therapies directed against P. aeruginosa infections. To examine the eligibility of phage KPP10 as a therapeutic phage, its genome was analyzed. The genomic DNA was shown to be 88,322 bp long, with 158 open reading frames (ORFs), and three tRNA genes were predicted. No ORF-encoded pathogenicity or lysogenization factor was predicted. A comparative genomic analysis revealed that phage KPP10, together with phage PAK_P3, can be grouped as a new type of lytic phage infecting P. aeruginosa. Phage KPP10 is considered to be suitable for therapeutic purposes because it is a lytic phage without ORF-encoded pathogenicity or a lysogenization factors.
AB - Bacteriophage (phage) KPP10 has been used in experimental phage therapies directed against P. aeruginosa infections. To examine the eligibility of phage KPP10 as a therapeutic phage, its genome was analyzed. The genomic DNA was shown to be 88,322 bp long, with 158 open reading frames (ORFs), and three tRNA genes were predicted. No ORF-encoded pathogenicity or lysogenization factor was predicted. A comparative genomic analysis revealed that phage KPP10, together with phage PAK_P3, can be grouped as a new type of lytic phage infecting P. aeruginosa. Phage KPP10 is considered to be suitable for therapeutic purposes because it is a lytic phage without ORF-encoded pathogenicity or a lysogenization factors.
KW - Bacteriophage
KW - Genomes
KW - Pseudomonas aeruginosa
KW - Therapeutic phage
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U2 - 10.1007/s00705-011-1210-x
DO - 10.1007/s00705-011-1210-x
M3 - Article
C2 - 22218962
AN - SCOPUS:84859103490
SN - 0304-8608
VL - 157
SP - 733
EP - 738
JO - Archives of Virology
JF - Archives of Virology
IS - 4
ER -