TY - JOUR
T1 - Genetic diversity of Mycobacterium tuberculosis Central Asian Strain isolates from Nepal and comparison with neighboring countries
AU - Shah, Yogendra
AU - Poudel, Ajay
AU - Maharjan, Bhagwan
AU - Thapa, Jeewan
AU - Yamaguchi, Tomoyuki
AU - Diab, Hassan Mahmoud
AU - Pandey, Basu Dev
AU - Solo, Eddie
AU - Isoda, Norikazu
AU - Suzuki, Yasuhiko
AU - Nakajima, Chie
N1 - Funding Information:
Funding: This study was supported by the Leading Program ‘Fostering Global Leaders in Veterinary Science toward Contributing to ‘One Health’’ from Ministry of Education, Culture, Sports, Science and Technology, Japan (MEXT), U.S.-Japan Cooperative Medical Science Programs from Japan Agency for Medical Research and development (AMED), Japanese Society for the Promotion of Science (JSPS) ROMPAKU Program, MEXT/ JSPS KAKENHI Grant Number 15K08724 and Research Program on Emerging and Re-emerging Infectious Diseases from AMED to CN.
Publisher Copyright:
© 2018 The Author(s).
PY - 2019/4/1
Y1 - 2019/4/1
N2 - Background Multidrug-resistant tuberculosis (MDR-TB) is an emerging threat for successful tuberculosis control worldwide. Central Asian Strain (CAS) has been reported as one of the dominant families contributing to MDR-TB in South Asia including Nepal, India and Pakistan. The aim of this study was to better understand the genetic characteristics of MDR-TB CAS family isolates circulating in Nepal and compare the results with neighboring countries. Methods A total of 145 MDR-TB CAS family isolates collected in Nepal from 2008 to 2013 were analyzed by spoligotyping and mycobacterial interspersed repetitive units-variable number of tandem repeats (MIRU-VNTR) analysis. In addition, we compared these data with published data from India and Pakistan to investigate a possible epidemiological link via construction of a minimum spanning tree (MST). Results Spoligotyping analysis exhibited CAS1-Delhi SIT26 (n=60) as the predominant lineage among the MDR-TB CAS family in all three countries. However, the combined analysis with spoligotyping and MIRU-VNTR further discriminated 60 isolates into 49 different types and 5 clusters. Each cluster was composed of 14 isolates with a clustering rate of 23.3%, suggesting ongoing transmissions. Based on MST data from neighboring countries, we elucidated an evolutionary relationship between the two countries, Nepal and India, which could be explained by their open border. Conclusion This study identified the evolutionary relationships among MDR-TB CAS1-Delhi subfamily isolates from Nepal and those from neighboring countries.
AB - Background Multidrug-resistant tuberculosis (MDR-TB) is an emerging threat for successful tuberculosis control worldwide. Central Asian Strain (CAS) has been reported as one of the dominant families contributing to MDR-TB in South Asia including Nepal, India and Pakistan. The aim of this study was to better understand the genetic characteristics of MDR-TB CAS family isolates circulating in Nepal and compare the results with neighboring countries. Methods A total of 145 MDR-TB CAS family isolates collected in Nepal from 2008 to 2013 were analyzed by spoligotyping and mycobacterial interspersed repetitive units-variable number of tandem repeats (MIRU-VNTR) analysis. In addition, we compared these data with published data from India and Pakistan to investigate a possible epidemiological link via construction of a minimum spanning tree (MST). Results Spoligotyping analysis exhibited CAS1-Delhi SIT26 (n=60) as the predominant lineage among the MDR-TB CAS family in all three countries. However, the combined analysis with spoligotyping and MIRU-VNTR further discriminated 60 isolates into 49 different types and 5 clusters. Each cluster was composed of 14 isolates with a clustering rate of 23.3%, suggesting ongoing transmissions. Based on MST data from neighboring countries, we elucidated an evolutionary relationship between the two countries, Nepal and India, which could be explained by their open border. Conclusion This study identified the evolutionary relationships among MDR-TB CAS1-Delhi subfamily isolates from Nepal and those from neighboring countries.
KW - CAS1-Delhi
KW - MIRU-VNTR
KW - MST
KW - multidrug-resistant TB
KW - Nepal
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U2 - 10.1093/trstmh/try136
DO - 10.1093/trstmh/try136
M3 - Article
C2 - 30668857
AN - SCOPUS:85063712554
SN - 0035-9203
VL - 113
SP - 203
EP - 211
JO - Transactions of the Royal Society of Tropical Medicine and Hygiene
JF - Transactions of the Royal Society of Tropical Medicine and Hygiene
IS - 4
ER -
