Geometrical structure of honeycomb TCP to control dental pulp-derived cell differentiation

Kiyofumi Takabatake, Hidetsugu Tsujigiwa, Keisuke Nakano, Yasunori Inada, Shan Qiusheng, Hotaka Kawai, Shintaro Sukegawa, Shigeko Fushimi, Hitoshi Nagatsuka

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


Recently, dental pulp has been attracting attention as a promising source of multipotent mesenchymal stem cells (MSCs) for various clinical applications of regeneration fields. To date, we have succeeded in establishing rat dental pulp-derived cells showing the characteristics of odontoblasts under in vitro conditions. We named them Tooth matrix-forming, GFP rat-derived Cells (TGC). However, though TGC form massive dentin-like hard tissues under in vivo conditions, this does not lead to the induction of polar odontoblasts. Focusing on the importance of the geometrical structure of an artificial biomaterial to induce cell differentiation and hard tissue formation, we previously have succeeded in developing a new biomaterial, honeycomb tricalcium phosphate (TCP) scaffold with through-holes of various diameters. In this study, to induce polar odontoblasts, TGC were induced to form odontoblasts using honeycomb TCP that had various hole diameters (75, 300, and 500 µm) as a scaffold. The results showed that honeycomb TCP with 300-µm hole diameters (300TCP) differentiated TGC into polar odontoblasts that were DSP positive. Therefore, our study indicates that 300TCP is an appropriate artificial biomaterial for dentin regeneration.

Original languageEnglish
Article number5155
Pages (from-to)1-10
Number of pages10
Issue number22
Publication statusPublished - Nov 2 2020


  • Dental pulp
  • Dentinformation
  • Geometrical structure
  • HoneycombTCP
  • Matrix formation
  • Scaffold

ASJC Scopus subject areas

  • Materials Science(all)


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