Girds 'n' cleeks o' cytokinesis: Microtubule sticks and contractile hoops in cell division

David M. Glover, Luisa Capalbo, Pier Paolo D'Avino, Melanie K. Gatt, Matthew S. Savoian, Tetsuya Takeda

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)


Microtubules maintain an intimate relationship with the rings of anillin, septins and actomyosin filaments throughout cytokinesis. In Drosophila, peripheral microtubules emanating from the spindle poles contact the equatorial cell cortex to deliver the signal that initiates formation of the cytokinetic furrow. Mutations that affect microtubule stability lead to ectopic furrowing because peripheral microtubules contact inappropriate cortical sites. The PAV-KLP (Pavarotti-kinesin-like protein)/RacGAP50C (where GAP is GTPase-activating protein) centralspindlin complex moves towards the plus ends of microtubules to reach the cell equator. When RacGAP50C is tethered to the cell membrane, furrowing initiates at multiple non-equatorial sites, indicating that mis-localization of this single molecule is sufficient to promote furrowing. Furrow formation and ingression requires RhoA activation by the RhoGEF (guanine-nucleotide-exchange factor) Pebble, which interacts with RacGAP50C. RacGAP50C also binds anillin, which associates with actin, myosin and septins. Thus RacGAP50C plays a pivotal role during furrow formation by activating RhoA and linking the peripheral microtubules with the nascent rings through its interaction with anillin.

Original languageEnglish
Pages (from-to)400-404
Number of pages5
JournalBiochemical Society Transactions
Issue number3
Publication statusPublished - Jun 2008
Externally publishedYes


  • Anillin
  • Central-spindle microtubule
  • Centralspindlin
  • Cytokinesis
  • Rac GTPase-activating protein (RacGAP)
  • Rho guanine-nucleotide-exchange factor (RhoGEF)

ASJC Scopus subject areas

  • Biochemistry


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