Glioma and temozolomide induced alterations in gut microbiome

Anthony Patrizz, Antonio Dono, Soheil Zorofchian, Gabriella Hines, Takeshi Takayasu, Nuruddin Husein, Yoshihiro Otani, Octavio Arevalo, H. Alex Choi, Jude Savarraj, Nitin Tandon, Bhanu P. Ganesh, Balveen Kaur, Louise D. McCullough, Leomar Y. Ballester, Yoshua Esquenazi

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)


The gut microbiome is fundamental in neurogenesis processes. Alterations in microbial constituents promote inflammation and immunosuppression. Recently, in immune-oncology, specific microbial taxa have been described to enhance the effects of therapeutic modalities. However, the effects of microbial dysbiosis on glioma are still unknown. The aim of this study was to explore the effects of glioma development and Temozolomide (TMZ) on fecal microbiome in mice and humans. C57BL/6 mice were implanted with GL261/Sham and given TMZ/Saline. Fecal samples were collected longitudinally and analyzed by 16S rRNA sequencing. Fecal samples were collected from healthy controls as well as glioma patients at diagnosis, before and after chemoradiation. Compared to healthy controls, mice and glioma patients demonstrated significant differences in beta diversity, Firmicutes/Bacteroides (F/B) ratio, and increase of Verrucomicrobia phylum and Akkermansia genus. These changes were not observed following TMZ in mice. TMZ treatment in the non-tumor bearing mouse-model diminished the F/B ratio, increase Muribaculaceae family and decrease Ruminococcaceae family. Nevertheless, there were no changes in Verrucomicrobia/Akkermansia. Glioma development leads to gut dysbiosis in a mouse-model, which was not observed in the setting of TMZ. These findings seem translational to humans and warrant further study.

Original languageEnglish
Article number21002
JournalScientific reports
Issue number1
Publication statusPublished - Dec 2020
Externally publishedYes

ASJC Scopus subject areas

  • General


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