Glycerol kinase from Cellulomonas sp. NT3060 which has high resistance against preservatives

Atsushi Sogabe; Masao Kitabayashi; Kenichi Morishima; Miyoko Furukawa; Takashi Hatta; Yasuhisa Fukuda; Hiroshi Nishise; Masanori Oka; Takashi Tamura; Kenji Inagaki

Glycerol kinase from Cellulomonas sp. NT3060 which has high resistance against preservatives

Atsushi Sogabe, Masao Kitabayashi, Kenichi Morishima, Miyoko Furukawa, Takashi Hatta, Yasuhisa Fukuda, Hiroshi Nishise, Masanori Oka, Takashi Tamura, Kenji Inagaki

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

Glycerol kinase is suitable for clinical diagnostic use in levels of neutral fat. Glycerol kinase from Cellulomonas sp. NT3060 shows good stability against several preservatives such as N-methylisothiazolone and imidazolidinylurea. We have cloned glpK gene from Cellulomonas sp. NT3060. This glpK gene is composed of 1,518 bp nucleotides and encodes 505 amino acid residues. The glpK gene was expressed in Escherichia coli and Cellulomonas sp. glycerol kinase was purified to homogeneity by column chromatog-raphy. The glycerol kinase exhibited high tolerance to various preservatives compared to other glycerol kinases. This tolerance was found to be favorable feature as diagnostic reagent for quantity of triglyceride.

Original language	English
Pages (from-to)	402-409
Number of pages	8
Journal	Seibutsu-kogaku Kaishi
Volume	92
Issue number	8
Publication status	Published - 2014

Keywords

Glycerol kinase
Preservative tolerance
Triglyceride measurement

ASJC Scopus subject areas

Biotechnology
Food Science
Applied Microbiology and Biotechnology

Cite this

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title = "Glycerol kinase from Cellulomonas sp. NT3060 which has high resistance against preservatives",

abstract = "Glycerol kinase is suitable for clinical diagnostic use in levels of neutral fat. Glycerol kinase from Cellulomonas sp. NT3060 shows good stability against several preservatives such as N-methylisothiazolone and imidazolidinylurea. We have cloned glpK gene from Cellulomonas sp. NT3060. This glpK gene is composed of 1,518 bp nucleotides and encodes 505 amino acid residues. The glpK gene was expressed in Escherichia coli and Cellulomonas sp. glycerol kinase was purified to homogeneity by column chromatog-raphy. The glycerol kinase exhibited high tolerance to various preservatives compared to other glycerol kinases. This tolerance was found to be favorable feature as diagnostic reagent for quantity of triglyceride.",

keywords = "Glycerol kinase, Preservative tolerance, Triglyceride measurement",

author = "Atsushi Sogabe and Masao Kitabayashi and Kenichi Morishima and Miyoko Furukawa and Takashi Hatta and Yasuhisa Fukuda and Hiroshi Nishise and Masanori Oka and Takashi Tamura and Kenji Inagaki",

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language = "English",

volume = "92",

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T1 - Glycerol kinase from Cellulomonas sp. NT3060 which has high resistance against preservatives

AU - Sogabe, Atsushi

AU - Kitabayashi, Masao

AU - Morishima, Kenichi

AU - Furukawa, Miyoko

AU - Hatta, Takashi

AU - Fukuda, Yasuhisa

AU - Nishise, Hiroshi

AU - Oka, Masanori

AU - Tamura, Takashi

AU - Inagaki, Kenji

PY - 2014

Y1 - 2014

N2 - Glycerol kinase is suitable for clinical diagnostic use in levels of neutral fat. Glycerol kinase from Cellulomonas sp. NT3060 shows good stability against several preservatives such as N-methylisothiazolone and imidazolidinylurea. We have cloned glpK gene from Cellulomonas sp. NT3060. This glpK gene is composed of 1,518 bp nucleotides and encodes 505 amino acid residues. The glpK gene was expressed in Escherichia coli and Cellulomonas sp. glycerol kinase was purified to homogeneity by column chromatog-raphy. The glycerol kinase exhibited high tolerance to various preservatives compared to other glycerol kinases. This tolerance was found to be favorable feature as diagnostic reagent for quantity of triglyceride.

AB - Glycerol kinase is suitable for clinical diagnostic use in levels of neutral fat. Glycerol kinase from Cellulomonas sp. NT3060 shows good stability against several preservatives such as N-methylisothiazolone and imidazolidinylurea. We have cloned glpK gene from Cellulomonas sp. NT3060. This glpK gene is composed of 1,518 bp nucleotides and encodes 505 amino acid residues. The glpK gene was expressed in Escherichia coli and Cellulomonas sp. glycerol kinase was purified to homogeneity by column chromatog-raphy. The glycerol kinase exhibited high tolerance to various preservatives compared to other glycerol kinases. This tolerance was found to be favorable feature as diagnostic reagent for quantity of triglyceride.

KW - Glycerol kinase

KW - Preservative tolerance

KW - Triglyceride measurement

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JO - Seibutsu-kogaku Kaishi

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Glycerol kinase from Cellulomonas sp. NT3060 which has high resistance against preservatives

Abstract

Keywords

ASJC Scopus subject areas

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