TY - JOUR
T1 - Glycocalyx damage estimated using colloidal iron staining
AU - Zhang, Daoxin
AU - Isaka, Yoshitaka
AU - Imamura, Ryoichi
AU - Ichimaru, Naotsugu
AU - Shi, Yi
AU - Imai, Enyu
AU - Tian, Ye
AU - Ohtsuka, Aiji
AU - Takahara, Shiro
PY - 2008
Y1 - 2008
N2 - Anionic constituents in the peritubular capillary basement membranes and the glomerular endothelial cells have been demonstrated to function as a size- and charge-selective barrier. Cationic colloidal iron staining of human biopsy specimens revealed a glycocalyx on the surface of the glomerular basement membrane (GBM), peritubular capillary (PTC) endothelial cells, and brush border of the tubular epithelial cells of normal kidney. However, the glycocalyx was abolished in the PTC wall of C4d-positive acute humoral rejected kidney, and in the GBM as well as the PTC wall of a chronic, allograft, nephropathy kidney. In addition, cyclosporine eliminated the glycocalyx in the PTC wall, while treatment with heparin inhibited the elimination of the PTC glycocalyx. In conclusion, the glycocalyx on the surface of the GBM and PTC is an important component in the endothelial cell barrier.
AB - Anionic constituents in the peritubular capillary basement membranes and the glomerular endothelial cells have been demonstrated to function as a size- and charge-selective barrier. Cationic colloidal iron staining of human biopsy specimens revealed a glycocalyx on the surface of the glomerular basement membrane (GBM), peritubular capillary (PTC) endothelial cells, and brush border of the tubular epithelial cells of normal kidney. However, the glycocalyx was abolished in the PTC wall of C4d-positive acute humoral rejected kidney, and in the GBM as well as the PTC wall of a chronic, allograft, nephropathy kidney. In addition, cyclosporine eliminated the glycocalyx in the PTC wall, while treatment with heparin inhibited the elimination of the PTC glycocalyx. In conclusion, the glycocalyx on the surface of the GBM and PTC is an important component in the endothelial cell barrier.
KW - Chronic allograft nephropathy
KW - Cyclosporine
KW - Glycocalyx
KW - Humoral rejection
KW - Transplantation
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U2 - 10.3727/000000008783907134
DO - 10.3727/000000008783907134
M3 - Article
C2 - 18468245
AN - SCOPUS:42249113679
SN - 0963-6897
VL - 17
SP - 159
EP - 163
JO - Cell Transplantation
JF - Cell Transplantation
IS - 1-2
ER -