TY - JOUR
T1 - Golgi apparatus casein kinase phosphorylates bioactive Ser-6 of bone morphogenetic protein 15 and growth and differentiation factor 9
AU - Tibaldi, Elena
AU - Arrigoni, Giorgio
AU - Martinez, Heather M.
AU - Inagaki, Kenichi
AU - Shimasaki, Shunichi
AU - Pinna, Lorenzo A.
N1 - Funding Information:
This work was supported by grants from AIRC and the European Commission (PRO-KINASERESEARCH 503467) to L.A. Pinna and from NIH RO1 HD41494 and NIH U54 HD012303 to S. Shimasaki.
PY - 2010/2
Y1 - 2010/2
N2 - Bone morphogenetic protein-15 (BMP-15) and growth and differentiation factor-9 (GDF-9) are oocyte-secreted factors that play essential roles in human folliculogenesis and ovulation. Their bioactivity is tightly regulated through phosphorylation, likely to occur within the Golgi apparatus of the secretory pathway. Here we show that Golgi apparatus casein kinase (G-CK) catalyzes the phosphorylation of rhBMP-15 and rhGDF-9. rhBMP-15, in particular, is an excellent substrate for G-CK. In each protein a single residue is phosphorylated by G-CK, corresponding to the serine residue at the sixth position of the mature region of both rhBMP-15 and rhGDF-9, whose phosphorylation is required for biological activity.
AB - Bone morphogenetic protein-15 (BMP-15) and growth and differentiation factor-9 (GDF-9) are oocyte-secreted factors that play essential roles in human folliculogenesis and ovulation. Their bioactivity is tightly regulated through phosphorylation, likely to occur within the Golgi apparatus of the secretory pathway. Here we show that Golgi apparatus casein kinase (G-CK) catalyzes the phosphorylation of rhBMP-15 and rhGDF-9. rhBMP-15, in particular, is an excellent substrate for G-CK. In each protein a single residue is phosphorylated by G-CK, corresponding to the serine residue at the sixth position of the mature region of both rhBMP-15 and rhGDF-9, whose phosphorylation is required for biological activity.
KW - Golgi-casein kinase
KW - Oocyte-secreted factor
KW - Phosphorylation
UR - http://www.scopus.com/inward/record.url?scp=77649271525&partnerID=8YFLogxK
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U2 - 10.1016/j.febslet.2009.12.052
DO - 10.1016/j.febslet.2009.12.052
M3 - Article
C2 - 20067794
AN - SCOPUS:77649271525
SN - 0014-5793
VL - 584
SP - 801
EP - 805
JO - FEBS Letters
JF - FEBS Letters
IS - 4
ER -