Green Tea Catechins, (−)-Catechin Gallate, and (−)-Gallocatechin Gallate are Potent Inhibitors of ABA-Induced Stomatal Closure

Stomatal movement is indispensable for plant growth and survival in response to environmental stimuli. Cytosolic Ca²⁺ elevation plays a crucial role in ABA-induced stomatal closure during drought stress; however, to what extent the Ca²⁺ movement across the plasma membrane from the apoplast to the cytosol contributes to this process still needs clarification. Here the authors identify (−)-catechin gallate (CG) and (−)-gallocatechin gallate (GCG), components of green tea, as inhibitors of voltage-dependent K⁺ channels which regulate K⁺ fluxes in Arabidopsis thaliana guard cells. In Arabidopsis guard cells CG/GCG prevent ABA-induced: i) membrane depolarization; ii) activation of Ca²⁺ permeable cation (I_Ca) channels; and iii) cytosolic Ca²⁺ transients. In whole Arabidopsis plants co-treatment with CG/GCG and ABA suppressed ABA-induced stomatal closure and surface temperature increase. Similar to ABA, CG/GCG inhibited stomatal closure is elicited by the elicitor peptide, flg22 but has no impact on dark-induced stomatal closure or light- and fusicoccin-induced stomatal opening, suggesting that the inhibitory effect of CG/GCG is associated with Ca²⁺-related signaling pathways. This study further supports the crucial role of I_Ca channels of the plasma membrane in ABA-induced stomatal closure. Moreover, CG and GCG represent a new tool for the study of abiotic or biotic stress-induced signal transduction pathways.