Heat stress is a potent stimulus for enhancing rescue efficiency of recombinant Borna disease virus

Shohei Kojima, Tomoyuki Honda, Yusuke Matsumoto, Keizo Tomonaga

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)


Recently developed vector systems based on Borna disease virus (BDV) hold promise as platforms for efficient and stable gene delivery to the central nervous system (CNS). However, because it currently takes several weeks to rescue recombinant BDV (rBDV), an improved rescue procedure would enhance the utility of this system. Heat stress reportedly enhances the rescue efficiency of other recombinant viruses. Here, heat stress was demonstrated to increase the amount of BDV genome in persistently BDV-infected cells without obvious cytotoxicity. Further analyses suggested that the effect of heat stress on BDV infection is not caused by an increase in the activity of BDV polymerase. More cells in which BDV replication occurs were obtained in the initial phase of rBDV rescue by using heat stress than when it was not used. Thus, heat stress is a useful improvement on the published rescue procedure for rBDV. The present findings may accelerate the practical use of BDV vector systems in basic science and the clinic and thus enable broader adoption of this viral vector, which is uniquely suited for gene delivery to the CNS.

Original languageEnglish
Pages (from-to)636-642
Number of pages7
Issue number11
Publication statusPublished - Nov 1 2014
Externally publishedYes


  • Animal RNA virus
  • Borna disease virus
  • Heat stress
  • Viral vector

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Virology


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