TY - JOUR
T1 - Hematopoietic progenitor cells from allogeneic bone marrow transplant donors circulate in the very early post-transplant period
AU - Katayama, Y.
AU - Mahmut, N.
AU - Takimoto, H.
AU - Maeda, Y.
AU - Yano, T.
AU - Kojima, K.
AU - Azuma, T.
AU - Hara, M.
AU - Imajyo, K.
AU - Takahashi, S.
AU - Kai, T.
AU - Ohno, Y.
AU - Miyamoto, T.
AU - Nagafuji, K.
AU - Matsue, K.
AU - Takenaka, K.
AU - Teshima, T.
AU - Shinagawa, K.
AU - Ishimaru, F.
AU - Omoto, E.
AU - Harada, M.
N1 - Funding Information:
This work was supported in part by grants-in-aid from the Ministry of Education, Science and Culture (06454348) and for Cancer Research from the Ministry of Health and Welfare, and a Research Grant for Immunology, Allergy and Organ Transplant, Ministry of Health and Welfare. We thank Ms Eiko Furuta and other co-medical staff (Hematology section, Ehime Prefectural Central Hospital, Ehime, Japan) for their kind assistance. We also thank Dr Yoshinobu Matsuo (Fujisaki Cell Center, Hayashibara Biochemical Labs, Inc, Okayama, Japan) for preparation of the Figures. We gratefully acknowledge Dr Kazuma Ikeda (Second Department of Internal Medicine, Okayama University Medical School, Okayama, Japan) for his kind advice and Mrs Chizu Katayama for her excellent technical assistance.
PY - 1999
Y1 - 1999
N2 - Despite the therapeutic efficacy of allogeneic bone marrow transplantation (allo-BMT), circulating hematopoietic progenitor cells after bone marrow transplantation have not been well characterized. In the present study, we focused on these 'post-transplant circulating progenitor cells (PTCPC)' which may be on their way to bone marrow. We analyzed the number of myeloid progenitor cells (CFU-GM) per 10 ml of peripheral blood (PB) on days 0 (just before transplantation), 1 (8-15 h after the completion of transplantation), 2, 3, 5, 7, 10, 14, 17, 21, 28 and 35 after allo-BMT in five transplant patients using a standard methylcellulose assay. In addition, high proliferative potential colony-forming cells (HPP-CFC) of the harvested donor bone marrow (BM) and day 1 PB of recipients were assayed in five patients. The origin of HPP-CFC from day 1 PB was analyzed by polymerase chain reaction of a DNA region containing a variable number of tandem repeats. The replating potential of these HPP-CFC was evaluated by a secondary colony assay. The proportion of CD38(negative) cells among CD34+ cells in the harvested BM and day 1 PB was evaluated by two-color flow cytometric analysis. The number of CFU-GM on day 1 ranged from 6 to 73/10 ml PB, and became undetectable on day 5. The reappearance of PTCPC was observed on day 14, along with hematopoietic recovery. The proportion of HPP-CFC among myeloid colonies from day 1 PB was significantly higher than that from harvested BM (44.3 ± 10.4% vs 11.3 ± 2.1%, respectively, n = 5, P = 0.0030). These HPP-CFC from day 1 PB were confirmed to be of donor origin. More than 90% of these HPP-CFC had replating potential. Two-color flow cytometric analysis revealed that the proportion of CD34+CD38(negative) cells was significantly higher in day 1 PB than in the harvested BM (61.0 ± 16.5% vs 9.3 ± 3.5%, respectively, n = 7, P = 0.0002). These observations suggest that both primitive and committed transplanted myeloid progenitor cells may circulate in the very early period following allo-BMT.
AB - Despite the therapeutic efficacy of allogeneic bone marrow transplantation (allo-BMT), circulating hematopoietic progenitor cells after bone marrow transplantation have not been well characterized. In the present study, we focused on these 'post-transplant circulating progenitor cells (PTCPC)' which may be on their way to bone marrow. We analyzed the number of myeloid progenitor cells (CFU-GM) per 10 ml of peripheral blood (PB) on days 0 (just before transplantation), 1 (8-15 h after the completion of transplantation), 2, 3, 5, 7, 10, 14, 17, 21, 28 and 35 after allo-BMT in five transplant patients using a standard methylcellulose assay. In addition, high proliferative potential colony-forming cells (HPP-CFC) of the harvested donor bone marrow (BM) and day 1 PB of recipients were assayed in five patients. The origin of HPP-CFC from day 1 PB was analyzed by polymerase chain reaction of a DNA region containing a variable number of tandem repeats. The replating potential of these HPP-CFC was evaluated by a secondary colony assay. The proportion of CD38(negative) cells among CD34+ cells in the harvested BM and day 1 PB was evaluated by two-color flow cytometric analysis. The number of CFU-GM on day 1 ranged from 6 to 73/10 ml PB, and became undetectable on day 5. The reappearance of PTCPC was observed on day 14, along with hematopoietic recovery. The proportion of HPP-CFC among myeloid colonies from day 1 PB was significantly higher than that from harvested BM (44.3 ± 10.4% vs 11.3 ± 2.1%, respectively, n = 5, P = 0.0030). These HPP-CFC from day 1 PB were confirmed to be of donor origin. More than 90% of these HPP-CFC had replating potential. Two-color flow cytometric analysis revealed that the proportion of CD34+CD38(negative) cells was significantly higher in day 1 PB than in the harvested BM (61.0 ± 16.5% vs 9.3 ± 3.5%, respectively, n = 7, P = 0.0002). These observations suggest that both primitive and committed transplanted myeloid progenitor cells may circulate in the very early period following allo-BMT.
KW - Allo-BMT
KW - CD34CD38 cells
KW - Circulating progenitor cells
KW - HPP-CFC
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U2 - 10.1038/sj.bmt.1701638
DO - 10.1038/sj.bmt.1701638
M3 - Article
C2 - 10218841
AN - SCOPUS:0032950437
SN - 0268-3369
VL - 23
SP - 659
EP - 665
JO - Bone Marrow Transplantation
JF - Bone Marrow Transplantation
IS - 7
ER -