Hepatocyte growth factor induces differentiation of adult rat bone marrow cells into a hepatocyte lineage in vitro

Seh Hoon Oh; Masahiro Miyazaki; Hirosuke Kouchi; Yusuke Inoue; Masakiyo Sakaguchi; Toshiya Tsuji; Nobuyuki Shima; Kanji Higashio; Masayoshi Namba

doi:10.1006/bbrc.2000.3985

Hepatocyte growth factor induces differentiation of adult rat bone marrow cells into a hepatocyte lineage in vitro

Seh Hoon Oh, Masahiro Miyazaki, Hirosuke Kouchi, Yusuke Inoue, Masakiyo Sakaguchi, Toshiya Tsuji, Nobuyuki Shima, Kanji Higashio, Masayoshi Namba

Research output: Contribution to journal › Article › peer-review

206 Citations (Scopus)

Abstract

Bone marrow (BM) cells originally include alphafetoprotein (AFP)- and c-Met [a receptor for hepatocyte growth factor (HGF)]-expressing cells. In vitro treatment of BM cells with HGF induced albumin-expressing hepatocyte-like cells. Furthermore, those hepatocyte-like cells expressed cytokeratins 8 and 18, which are typically expressed in normal adult hepatocymes. These findings demonstrate that BM cells include AFP-expressing hepatic progenitor cells that can be differentiated into hepatocytes by HGF in culture, indicating that such cultures are useful resources for cell transplantation therapy for liver diseases.

Original language	English
Pages (from-to)	500-504
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	279
Issue number	2
DOIs	https://doi.org/10.1006/bbrc.2000.3985
Publication status	Published - Dec 20 2000

Keywords

Bone marrow cells
Differentiation
Hepatocyte growth factor
Hepatocyte lineage

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1006/bbrc.2000.3985

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abstract = "Bone marrow (BM) cells originally include alphafetoprotein (AFP)- and c-Met [a receptor for hepatocyte growth factor (HGF)]-expressing cells. In vitro treatment of BM cells with HGF induced albumin-expressing hepatocyte-like cells. Furthermore, those hepatocyte-like cells expressed cytokeratins 8 and 18, which are typically expressed in normal adult hepatocymes. These findings demonstrate that BM cells include AFP-expressing hepatic progenitor cells that can be differentiated into hepatocytes by HGF in culture, indicating that such cultures are useful resources for cell transplantation therapy for liver diseases.",

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AU - Oh, Seh Hoon

AU - Miyazaki, Masahiro

AU - Kouchi, Hirosuke

AU - Inoue, Yusuke

AU - Sakaguchi, Masakiyo

AU - Tsuji, Toshiya

AU - Shima, Nobuyuki

AU - Higashio, Kanji

AU - Namba, Masayoshi

PY - 2000/12/20

Y1 - 2000/12/20

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AB - Bone marrow (BM) cells originally include alphafetoprotein (AFP)- and c-Met [a receptor for hepatocyte growth factor (HGF)]-expressing cells. In vitro treatment of BM cells with HGF induced albumin-expressing hepatocyte-like cells. Furthermore, those hepatocyte-like cells expressed cytokeratins 8 and 18, which are typically expressed in normal adult hepatocymes. These findings demonstrate that BM cells include AFP-expressing hepatic progenitor cells that can be differentiated into hepatocytes by HGF in culture, indicating that such cultures are useful resources for cell transplantation therapy for liver diseases.

KW - Bone marrow cells

KW - Differentiation

KW - Hepatocyte growth factor

KW - Hepatocyte lineage

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Hepatocyte growth factor induces differentiation of adult rat bone marrow cells into a hepatocyte lineage in vitro

Abstract

Keywords

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