Abstract
We have previously reported the presence of human β-defensin-2 (HBD-2), a peptide with antimicrobial and cytotoxic properties, in oral squamous cell carcinomas. The aim of the present study was to measure the concentration of HBD-2 in abnormal tissues such as oral squamous cell carcinomas. HBD-2 was extracted from tissue samples in the presence of retinoic acid and subjected to reversed-phase HPLC. The fraction representing peak 17 (P 17) was obtained by elution using a linear gradient of acetonitrile. Amino acid sequencing and homology studies were subsequently performed, and the molecular weight of P17 was calculated to be 4327.02 daltons. This molecular weight was consistent with HBD-2. The concentration of HBD-2 in the oral squamous cell carcinoma samples was 3.85±1.87 μg/mg which was much higher than in normal oral epithelium (0.04±0.02 μg/mg).
| Original language | English |
|---|---|
| Pages (from-to) | 2103-2107 |
| Number of pages | 5 |
| Journal | Anticancer research |
| Volume | 22 |
| Issue number | 4 |
| Publication status | Published - 2002 |
Keywords
- Defensin
- HBD-2
- Reversed-phase HPLC
- Squamous cell carcinoma
ASJC Scopus subject areas
- Oncology
- Cancer Research
