High frequency Agrobacterium-mediated transformation and plant regeneration via direct shoot formation from leaf explants in Beta vulgaris and Beta maritima

H. Hisano; Y. Kimoto; H. Hayakawa; J. Takeichi; T. Domae; R. Hashimoto; J. Abe; S. Asano; A. Kanazawa; Y. Shimamoto

doi:10.1007/s00299-004-0773-3

High frequency Agrobacterium-mediated transformation and plant regeneration via direct shoot formation from leaf explants in Beta vulgaris and Beta maritima

H. Hisano, Y. Kimoto, H. Hayakawa, J. Takeichi, T. Domae, R. Hashimoto, J. Abe, S. Asano, A. Kanazawa, Y. Shimamoto

Research output: Contribution to journal › Article › peer-review

29 Citations (Scopus)

Abstract

We have developed a new procedure for Agrobacterium-mediated transformation of plants in the genus Beta using shoot-base as the material for Agrobacterium infection. The frequency of regeneration from shoot bases was analyzed in seven accessions of sugarbeet (Beta vulgaris) and two accessions of B. maritima to select materials suitable for obtaining transformed plants. The frequency of transformation of the chosen accessions using Agrobacterium strain LBA4404 and selection on 150-mg/l kanamycin was found to be higher than that in previously published methods. Genomic DNA analysis and β-glucuronidase reporter assays showed that the transgene was inherited and expressed in subsequent generations. In our method, shoot bases are prepared by a simple procedure, and transformation does not involve the callus phase, thus minimizing the occurrence of somaclonal variations.

Original language	English
Pages (from-to)	910-918
Number of pages	9
Journal	Plant Cell Reports
Volume	22
Issue number	12
DOIs	https://doi.org/10.1007/s00299-004-0773-3
Publication status	Published - Jul 2004
Externally published	Yes

Keywords

Agrobacterium tumefaciens
Beta vulgaris
Regeneration
Shoot-base tissue
Transformation

ASJC Scopus subject areas

Agronomy and Crop Science
Plant Science

Access to Document

10.1007/s00299-004-0773-3

Cite this

Hisano, H., Kimoto, Y., Hayakawa, H., Takeichi, J., Domae, T., Hashimoto, R., Abe, J., Asano, S., Kanazawa, A., & Shimamoto, Y. (2004). High frequency Agrobacterium-mediated transformation and plant regeneration via direct shoot formation from leaf explants in Beta vulgaris and Beta maritima. Plant Cell Reports, 22(12), 910-918. https://doi.org/10.1007/s00299-004-0773-3

Hisano, H, Kimoto, Y, Hayakawa, H, Takeichi, J, Domae, T, Hashimoto, R, Abe, J, Asano, S, Kanazawa, A & Shimamoto, Y 2004, 'High frequency Agrobacterium-mediated transformation and plant regeneration via direct shoot formation from leaf explants in Beta vulgaris and Beta maritima', Plant Cell Reports, vol. 22, no. 12, pp. 910-918. https://doi.org/10.1007/s00299-004-0773-3

@article{f30deeb2c41e45228795e6b377dc2c63,

title = "High frequency Agrobacterium-mediated transformation and plant regeneration via direct shoot formation from leaf explants in Beta vulgaris and Beta maritima",

abstract = "We have developed a new procedure for Agrobacterium-mediated transformation of plants in the genus Beta using shoot-base as the material for Agrobacterium infection. The frequency of regeneration from shoot bases was analyzed in seven accessions of sugarbeet (Beta vulgaris) and two accessions of B. maritima to select materials suitable for obtaining transformed plants. The frequency of transformation of the chosen accessions using Agrobacterium strain LBA4404 and selection on 150-mg/l kanamycin was found to be higher than that in previously published methods. Genomic DNA analysis and β-glucuronidase reporter assays showed that the transgene was inherited and expressed in subsequent generations. In our method, shoot bases are prepared by a simple procedure, and transformation does not involve the callus phase, thus minimizing the occurrence of somaclonal variations.",

keywords = "Agrobacterium tumefaciens, Beta vulgaris, Regeneration, Shoot-base tissue, Transformation",

author = "H. Hisano and Y. Kimoto and H. Hayakawa and J. Takeichi and T. Domae and R. Hashimoto and J. Abe and S. Asano and A. Kanazawa and Y. Shimamoto",

year = "2004",

month = jul,

doi = "10.1007/s00299-004-0773-3",

language = "English",

volume = "22",

pages = "910--918",

journal = "Plant Cell Reports",

issn = "0721-7714",

publisher = "Springer Verlag",

number = "12",

}

TY - JOUR

T1 - High frequency Agrobacterium-mediated transformation and plant regeneration via direct shoot formation from leaf explants in Beta vulgaris and Beta maritima

AU - Hisano, H.

AU - Kimoto, Y.

AU - Hayakawa, H.

AU - Takeichi, J.

AU - Domae, T.

AU - Hashimoto, R.

AU - Abe, J.

AU - Asano, S.

AU - Kanazawa, A.

AU - Shimamoto, Y.

PY - 2004/7

Y1 - 2004/7

N2 - We have developed a new procedure for Agrobacterium-mediated transformation of plants in the genus Beta using shoot-base as the material for Agrobacterium infection. The frequency of regeneration from shoot bases was analyzed in seven accessions of sugarbeet (Beta vulgaris) and two accessions of B. maritima to select materials suitable for obtaining transformed plants. The frequency of transformation of the chosen accessions using Agrobacterium strain LBA4404 and selection on 150-mg/l kanamycin was found to be higher than that in previously published methods. Genomic DNA analysis and β-glucuronidase reporter assays showed that the transgene was inherited and expressed in subsequent generations. In our method, shoot bases are prepared by a simple procedure, and transformation does not involve the callus phase, thus minimizing the occurrence of somaclonal variations.

AB - We have developed a new procedure for Agrobacterium-mediated transformation of plants in the genus Beta using shoot-base as the material for Agrobacterium infection. The frequency of regeneration from shoot bases was analyzed in seven accessions of sugarbeet (Beta vulgaris) and two accessions of B. maritima to select materials suitable for obtaining transformed plants. The frequency of transformation of the chosen accessions using Agrobacterium strain LBA4404 and selection on 150-mg/l kanamycin was found to be higher than that in previously published methods. Genomic DNA analysis and β-glucuronidase reporter assays showed that the transgene was inherited and expressed in subsequent generations. In our method, shoot bases are prepared by a simple procedure, and transformation does not involve the callus phase, thus minimizing the occurrence of somaclonal variations.

KW - Agrobacterium tumefaciens

KW - Beta vulgaris

KW - Regeneration

KW - Shoot-base tissue

KW - Transformation

UR - http://www.scopus.com/inward/record.url?scp=3242718776&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=3242718776&partnerID=8YFLogxK

U2 - 10.1007/s00299-004-0773-3

DO - 10.1007/s00299-004-0773-3

M3 - Article

C2 - 15042407

AN - SCOPUS:3242718776

SN - 0721-7714

VL - 22

SP - 910

EP - 918

JO - Plant Cell Reports

JF - Plant Cell Reports

IS - 12

ER -

High frequency Agrobacterium-mediated transformation and plant regeneration via direct shoot formation from leaf explants in Beta vulgaris and Beta maritima

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this