TY - JOUR
T1 - High temperature-induced sterility in the female Nile tilapia, Oreochromis niloticus
AU - Pandit, Narayan Prasad
AU - Bhandari, Ramji Kumar
AU - Kobayashi, Yasuhisa
AU - Nakamura, Masaru
N1 - Funding Information:
This study was supported by Grants-in-Aid ( 23658166 ) from Japan Society for the Promotion of Science (JSPS) to MN. This study would not be possible without a contribution of Sesoko Station, Tropical Biosphere Research Center of the University of the Ryukyus. Authors thank Ms. Caitlin Jandegain, University of Missouri for invaluable comments on the manuscript. MN and NP designed experiment, NP performed experiment, YK, RB, and NP analyzed data and wrote the manuscript.
Publisher Copyright:
© 2015 Elsevier Inc.
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2015/3/1
Y1 - 2015/3/1
N2 - High temperature treatments induce germ cell loss in gonads of vertebrate animals, including fish. It could be a reliable source for induction of sterility if the treatments led to a permanent loss of germ cells. Here we report that heat treatment at 37. °C for 45-60. days caused a complete loss of germ cells in female Nile tilapia, Oreochromis niloticus, and that sterility was achieved in fish at all stages of their life cycle. Unlike previous observations, germ cells did not repopulate even after returning them to the water at control conditions suggesting permanent depletion of germ cells. Gonadal somatic cells immunopositive for 3β-hydroxysteroid dehydrogenase (3β-HSD) were clustered at one end of the germ cell depleted gonads close to the blood vessel. Serum level of testosterone, 11-ketotestosterone, and 17β-estradiol was significantly decreased in sterile fish compared to control. Body weight of sterile fish was higher than control fish at the end of experiment. Our observations of increased growth and permanent sterilization in the high temperature-treated fish suggest that this method could be an appropriate and eco-friendly tool for inducing sterility in fish with a higher thermal tolerance.
AB - High temperature treatments induce germ cell loss in gonads of vertebrate animals, including fish. It could be a reliable source for induction of sterility if the treatments led to a permanent loss of germ cells. Here we report that heat treatment at 37. °C for 45-60. days caused a complete loss of germ cells in female Nile tilapia, Oreochromis niloticus, and that sterility was achieved in fish at all stages of their life cycle. Unlike previous observations, germ cells did not repopulate even after returning them to the water at control conditions suggesting permanent depletion of germ cells. Gonadal somatic cells immunopositive for 3β-hydroxysteroid dehydrogenase (3β-HSD) were clustered at one end of the germ cell depleted gonads close to the blood vessel. Serum level of testosterone, 11-ketotestosterone, and 17β-estradiol was significantly decreased in sterile fish compared to control. Body weight of sterile fish was higher than control fish at the end of experiment. Our observations of increased growth and permanent sterilization in the high temperature-treated fish suggest that this method could be an appropriate and eco-friendly tool for inducing sterility in fish with a higher thermal tolerance.
KW - Germ cells
KW - High temperature
KW - Nile tilapia
KW - Sterility
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U2 - 10.1016/j.ygcen.2015.01.028
DO - 10.1016/j.ygcen.2015.01.028
M3 - Article
C2 - 25745814
AN - SCOPUS:84924674653
SN - 0016-6480
VL - 213
SP - 110
EP - 117
JO - General and Comparative Endocrinology
JF - General and Comparative Endocrinology
ER -