Highly efficient transgene-free targeted mutagenesis and single-stranded oligodeoxynucleotide-mediated precise knock-in in the industrial microalga Euglena gracilis using Cas9 ribonucleoproteins

Toshihisa Nomura, Komaki Inoue, Yukiko Uehara-Yamaguchi, Koji Yamada, Osamu Iwata, Kengo Suzuki, Keiichi Mochida

Research output: Contribution to journalEditorialpeer-review

27 Citations (Scopus)
Original languageEnglish
Pages (from-to)2032-2034
Number of pages3
JournalPlant Biotechnology Journal
Issue number11
Publication statusPublished - Nov 1 2019


  • CRISPR/Cas9
  • Euglena gracilis
  • Microalgae
  • genome editing
  • knock-in
  • ribonucleoproteins
  • single-stranded oligodeoxynucleotides
  • transgene free

ASJC Scopus subject areas

  • Biotechnology
  • Agronomy and Crop Science
  • Plant Science

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