Histatin as a synergistic stimulator with epidermal growth factor of rabbit chondrocyte proliferation

Yukitaka Murakami; Hideki Nagata; Satoshi Shizukuishi; Kazuhisa Nakashima; Tokutaro Okawa; Masaharu Takigawa; Akira Tsunemitsu

doi:10.1006/bbrc.1994.1038

Histatin as a synergistic stimulator with epidermal growth factor of rabbit chondrocyte proliferation

Yukitaka Murakami, Hideki Nagata, Satoshi Shizukuishi, Kazuhisa Nakashima, Tokutaro Okawa, Masaharu Takigawa, Akira Tsunemitsu

Research output: Contribution to journal › Article › peer-review

31 Citations (Scopus)

Abstract

Histatin 5 dose-dependently increased DNA synthesis in rabbit costal chondrocytes in culture. The level of DNA synthesis was stimulated by histatin 5 to about 4 times that of the control. The combination of histatin 5 and epidermal growth factor (EGF) increased the DNA synthesis to about 40 times that of the control while EGF alone stimulated it 15-fold, indicating synergistic stimulation by both factors. Enzyme-linked immunosorbent assay using anti-histatin 5 demonstrated that human serum contained histatin-like substances. These findings suggest that histatins play an important role in chondrocyte proliferation, presumably as a physiological modulator of the action of EGF.

Original language	English
Pages (from-to)	274-280
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	198
Issue number	1
DOIs	https://doi.org/10.1006/bbrc.1994.1038
Publication status	Published - Jan 15 1994
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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abstract = "Histatin 5 dose-dependently increased DNA synthesis in rabbit costal chondrocytes in culture. The level of DNA synthesis was stimulated by histatin 5 to about 4 times that of the control. The combination of histatin 5 and epidermal growth factor (EGF) increased the DNA synthesis to about 40 times that of the control while EGF alone stimulated it 15-fold, indicating synergistic stimulation by both factors. Enzyme-linked immunosorbent assay using anti-histatin 5 demonstrated that human serum contained histatin-like substances. These findings suggest that histatins play an important role in chondrocyte proliferation, presumably as a physiological modulator of the action of EGF.",

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AU - Takigawa, Masaharu

AU - Tsunemitsu, Akira

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Histatin as a synergistic stimulator with epidermal growth factor of rabbit chondrocyte proliferation

Abstract

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