@article{52af6926a70940bda7fab532459cbb89,
title = "HopAZ1, a type III effector of Pseudomonas amygdali pv. tabaci, induces a hypersensitive response in tobacco wildfire-resistant Nicotiana tabacum {\textquoteleft}N509{\textquoteright}",
abstract = "Pseudomonas amygdali pv. tabaci (formerly Pseudomonas syringae pv. tabaci; Pta) is a gram-negative bacterium that causes bacterial wildfire disease in Nicotiana tabacum. The pathogen establishes infections by using a type III secretion system to inject type III effector proteins (T3Es) into cells, thereby interfering with the host__s immune system. To counteract the effectors, plants have evolved disease-resistance genes and mechanisms to induce strong resistance on effector recognition. By screening a series of Pta T3E-deficient mutants, we have identified HopAZ1 as the T3E that induces disease resistance in N. tabacum {\textquoteleft}N509{\textquoteright}. Inoculation with the Pta ∆hopAZ1 mutant did not induce resistance to Pta in N509. We also found that the Pta ∆hopAZ1 mutant did not induce a hypersensitive response and promoted severe disease symptoms in N509. Furthermore, a C-terminal truncated HopAZ1 abolished HopAZ1-dependent cell death in N509. These results indicate that HopAZ1 is the avirulence factor that induces resistance to Pta by N509.",
keywords = "effector, hypersensitive responses, Pseudomonas syringae pv. tabaci, type III secretion system",
author = "Sachi Kashihara and Takafumi Nishimura and Yoshiteru Noutoshi and Mikihiro Yamamoto and Kazuhiro Toyoda and Yuki Ichinose and Hidenori Matsui",
note = "Funding Information: The authors thank the Leaf Tobacco Research Laboratory of Japan Tobacco Inc. for providing Pta 6605, tobacco cultivars, and wild tobacco plants. We also thank the National BioResource Project (NIG, Japan): Escherichia coli for providing E. coli S17-1. This work was supported in part by a Grants-in-Aid for Scientific Research (19H02956) from the Ministry of Education, Culture, Sports, Science and Technology of Japan. Funding Information: The authors thank the Leaf Tobacco Research Laboratory of Japan Tobacco Inc. for providing Pta 6605, tobacco cultivars, and wild tobacco plants. We also thank the National BioResource Project (NIG, Japan): for providing S17‐1. This work was supported in part by a Grants‐in‐Aid for Scientific Research (19H02956) from the Ministry of Education, Culture, Sports, Science and Technology of Japan. Escherichia coli E. coli Publisher Copyright: {\textcopyright} 2022 The Authors. Molecular Plant Pathology published by British Society for Plant Pathology and John Wiley & Sons Ltd.",
year = "2022",
month = jun,
doi = "10.1111/mpp.13198",
language = "English",
volume = "23",
pages = "885--894",
journal = "Molecular Plant Pathology",
issn = "1464-6722",
publisher = "Wiley-Blackwell",
number = "6",
}