TY - JOUR
T1 - Human albumin augmented airway inflammation induced by PM2.5 in NC/Nga mice
AU - Nagaoka, Kenjiro
AU - Ogino, Keiki
AU - Ogino, Noriyoshi
AU - Ito, Tatsuo
AU - Takemoto, Kei
AU - Ogino, Shihona
AU - Seki, Yuka
AU - Hamada, Hiroki
AU - Fujikura, Yoshihisa
N1 - Funding Information:
JSPS KAKENHI Grant, Grant/Award Number: 26670331, 18H03039 and 18K19681
Funding Information:
eosinophils in BALF was supported by the upregulation of mRNA of eotaxin 1 and 2, which are responsible for the expression of eosinophils in this study. Moreover, Th2 cytokines, such as IL-4, IL-5, and IL-13 are crucial for the pathogenesis of allergic asthma.27 The upregulation of mRNA for IL-13 in this study showed the involvement of IL-13 in the elevation of AHR.
Publisher Copyright:
© 2019 Wiley Periodicals, Inc.
Copyright:
Copyright 2019 Elsevier B.V., All rights reserved.
PY - 2019/7
Y1 - 2019/7
N2 - The synergic allergic inflammatory effects of particulate matter (PM) 2.5 and human albumin were investigated in NC/Nga mice, which are hypersensitive to mite allergens. PM2.5 or PM2.5 plus human albumin with aluminum oxide was injected twice intraperitoneally for sensitization. After 7 days, PM2.5 or PM2.5 plus human albumin was administered five times intranasally to mice for further sensitization. Subsequently, PM2.5 was administered as a challenge on the 11th day. On the 12th day, mice were examined for airway hyperresponsiveness (AHR), bronchoalveolar lavage fluid (BALF) cell count, mRNA expression of Th1, Th2 cytokines, chemokines, and mucus proteins (MUC5AC and MUC5B) in the lung tissue and histopathology. Although PM2.5 or human albumin alone did not induce allergic airway inflammation, simultaneous inoculation of PM2.5 and human albumin-induced airway inflammation showing increase in AHR, total BALF cell numbers, mRNA levels of IL-13, eotaxin 1, eotaxin 2, and MUC5AC, and anti-IG against human serum albumin. Inflammation was observed around the bronchus in PM2.5 plus human albumin-induced lungs. These results demonstrate that PM2.5 can induce allergic airway inflammation through the synergistic action with human albumin in NC/Nga mice.
AB - The synergic allergic inflammatory effects of particulate matter (PM) 2.5 and human albumin were investigated in NC/Nga mice, which are hypersensitive to mite allergens. PM2.5 or PM2.5 plus human albumin with aluminum oxide was injected twice intraperitoneally for sensitization. After 7 days, PM2.5 or PM2.5 plus human albumin was administered five times intranasally to mice for further sensitization. Subsequently, PM2.5 was administered as a challenge on the 11th day. On the 12th day, mice were examined for airway hyperresponsiveness (AHR), bronchoalveolar lavage fluid (BALF) cell count, mRNA expression of Th1, Th2 cytokines, chemokines, and mucus proteins (MUC5AC and MUC5B) in the lung tissue and histopathology. Although PM2.5 or human albumin alone did not induce allergic airway inflammation, simultaneous inoculation of PM2.5 and human albumin-induced airway inflammation showing increase in AHR, total BALF cell numbers, mRNA levels of IL-13, eotaxin 1, eotaxin 2, and MUC5AC, and anti-IG against human serum albumin. Inflammation was observed around the bronchus in PM2.5 plus human albumin-induced lungs. These results demonstrate that PM2.5 can induce allergic airway inflammation through the synergistic action with human albumin in NC/Nga mice.
KW - NC/Nga mice
KW - PM2.5
KW - allergic airway inflammation
KW - human albumin
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U2 - 10.1002/tox.22751
DO - 10.1002/tox.22751
M3 - Article
C2 - 30953400
AN - SCOPUS:85063928848
SN - 1520-4081
VL - 34
SP - 836
EP - 843
JO - Environmental Toxicology
JF - Environmental Toxicology
IS - 7
ER -
