Human serum immuno-reactive copper, zinc-superoxide dismutase assayed with an enzyme monoclonal immunosorbent in patients with digestive cancer

Shinji Oka; Keiki Ogino; Shinjiro Matsuura; Syunji Yoshimura; Kazunari Yamamoto; Yukinori Okazaki; Tadayoshi Takemoto; Nobuyasu Kato; Taizo Uda

doi:10.1016/0009-8981(89)90079-X

Human serum immuno-reactive copper, zinc-superoxide dismutase assayed with an enzyme monoclonal immunosorbent in patients with digestive cancer

Shinji Oka, Keiki Ogino, Shinjiro Matsuura, Syunji Yoshimura, Kazunari Yamamoto, Yukinori Okazaki, Tadayoshi Takemoto, Nobuyasu Kato, Taizo Uda

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

Two types of monoclonal antibodies, N4 and N6, were raised against human copper, zinc-superoxide dismutase (Cu, Zn-SOD). An enzyme-linked immunosorbent assay for Cu, Zn-SOD was developed in sandwich method using these monoclonal antibodies. The measurable assay range was 1.0-100 gmg/l and the coefficient of variation within and between series were 7.5% and 8.1%. Sera of 154 healthy control subjects displayed a low level (14 μg/l) of immunoreactive Cu, Zn-SOD with a logarithmic normal distribution. On the other hand, serum Cu, Zn-SOD levels were significantly elevated in patients with various digestive cancers.

Original language	English
Pages (from-to)	209-219
Number of pages	11
Journal	Clinica Chimica Acta
Volume	182
Issue number	2
DOIs	https://doi.org/10.1016/0009-8981(89)90079-X
Publication status	Published - Jun 30 1989
Externally published	Yes

Keywords

Digestive cancer
Enzyme-linked immunosorbent assay (ELISA)
Human copper, zinc-superoxide dismutase (Cu, Zn-SOD)
Monoclonal antibody

ASJC Scopus subject areas

Biochemistry
Clinical Biochemistry
Biochemistry, medical

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/0009-8981(89)90079-X

Cite this

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title = "Human serum immuno-reactive copper, zinc-superoxide dismutase assayed with an enzyme monoclonal immunosorbent in patients with digestive cancer",

abstract = "Two types of monoclonal antibodies, N4 and N6, were raised against human copper, zinc-superoxide dismutase (Cu, Zn-SOD). An enzyme-linked immunosorbent assay for Cu, Zn-SOD was developed in sandwich method using these monoclonal antibodies. The measurable assay range was 1.0-100 gmg/l and the coefficient of variation within and between series were 7.5% and 8.1%. Sera of 154 healthy control subjects displayed a low level (14 μg/l) of immunoreactive Cu, Zn-SOD with a logarithmic normal distribution. On the other hand, serum Cu, Zn-SOD levels were significantly elevated in patients with various digestive cancers.",

keywords = "Digestive cancer, Enzyme-linked immunosorbent assay (ELISA), Human copper, zinc-superoxide dismutase (Cu, Zn-SOD), Monoclonal antibody",

author = "Shinji Oka and Keiki Ogino and Shinjiro Matsuura and Syunji Yoshimura and Kazunari Yamamoto and Yukinori Okazaki and Tadayoshi Takemoto and Nobuyasu Kato and Taizo Uda",

year = "1989",

month = jun,

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doi = "10.1016/0009-8981(89)90079-X",

language = "English",

volume = "182",

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journal = "Clinica Chimica Acta",

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TY - JOUR

T1 - Human serum immuno-reactive copper, zinc-superoxide dismutase assayed with an enzyme monoclonal immunosorbent in patients with digestive cancer

AU - Oka, Shinji

AU - Ogino, Keiki

AU - Matsuura, Shinjiro

AU - Yoshimura, Syunji

AU - Yamamoto, Kazunari

AU - Okazaki, Yukinori

AU - Takemoto, Tadayoshi

AU - Kato, Nobuyasu

AU - Uda, Taizo

PY - 1989/6/30

Y1 - 1989/6/30

N2 - Two types of monoclonal antibodies, N4 and N6, were raised against human copper, zinc-superoxide dismutase (Cu, Zn-SOD). An enzyme-linked immunosorbent assay for Cu, Zn-SOD was developed in sandwich method using these monoclonal antibodies. The measurable assay range was 1.0-100 gmg/l and the coefficient of variation within and between series were 7.5% and 8.1%. Sera of 154 healthy control subjects displayed a low level (14 μg/l) of immunoreactive Cu, Zn-SOD with a logarithmic normal distribution. On the other hand, serum Cu, Zn-SOD levels were significantly elevated in patients with various digestive cancers.

AB - Two types of monoclonal antibodies, N4 and N6, were raised against human copper, zinc-superoxide dismutase (Cu, Zn-SOD). An enzyme-linked immunosorbent assay for Cu, Zn-SOD was developed in sandwich method using these monoclonal antibodies. The measurable assay range was 1.0-100 gmg/l and the coefficient of variation within and between series were 7.5% and 8.1%. Sera of 154 healthy control subjects displayed a low level (14 μg/l) of immunoreactive Cu, Zn-SOD with a logarithmic normal distribution. On the other hand, serum Cu, Zn-SOD levels were significantly elevated in patients with various digestive cancers.

KW - Digestive cancer

KW - Enzyme-linked immunosorbent assay (ELISA)

KW - Human copper, zinc-superoxide dismutase (Cu, Zn-SOD)

KW - Monoclonal antibody

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U2 - 10.1016/0009-8981(89)90079-X

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SN - 0009-8981

VL - 182

SP - 209

EP - 219

JO - Clinica Chimica Acta

JF - Clinica Chimica Acta

IS - 2

ER -

Human serum immuno-reactive copper, zinc-superoxide dismutase assayed with an enzyme monoclonal immunosorbent in patients with digestive cancer

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

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