Hyaluronan stimulates chondrogenic gene expression in human meniscus cells

Takaaki Tanaka, Takayuki Furumatsu, Shinichi Miyazawa, Masataka Fujii, Hiroto Inoue, Yuya Kodama, Toshifumi Ozaki

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)


Purpose/Aim of the Study: Inner meniscus cells have a chondrocytic phenotype, whereas outer meniscus cells have a fibroblastic phenotype. In this study, we examined the effect of hyaluronan on chondrocytic gene expression in human meniscus cells. Materials and Methods: Human meniscus cells were prepared from macroscopically intact lateral meniscus. Inner and outer meniscus cells were obtained from the inner and outer halves of the meniscus. The cells were stimulated with hyaluronan diluted in Dulbecco’s modified Eagle’s medium without serum to the desired concentration (0, 10, 100, and 1000 μg/mL) for 2–7 days. Cellular proliferation, migration, and polymerase chain reaction analyses were performed for the inner and outer cells separately. Meniscal samples perforated by a 2 mm diameter punch were maintained for 3 weeks in hyaluronan-supplemented medium and evaluated by histological analyses. Results: Hyaluronan increased the proliferation and migration of both meniscus cell types. Moreover, cellular counts at the surface of both meniscal tissue perforations were increased by hyaluronan treatments. In addition, hyaluronan stimulated α1(II) collagen expression in inner meniscus cells. Accumulation of type II collagen at the perforated surface of both meniscal samples was induced by hyaluronan treatment. Hyaluronan did not induce type I collagen accumulation around the injured site of the meniscus. Conclusion: Hyaluronan stimulated the proliferation and migration of meniscus cells. Our results suggest that hyaluronan may promote the healing potential of meniscus cells in damaged meniscal tissues.

Original languageEnglish
Pages (from-to)520-530
Number of pages11
JournalConnective Tissue Research
Issue number6
Publication statusPublished - Nov 2 2017


  • Chondrocytic gene expression
  • hyaluronan
  • inner meniscus
  • meniscus
  • outer meniscus
  • type II collagen

ASJC Scopus subject areas

  • Rheumatology
  • Biochemistry
  • Orthopedics and Sports Medicine
  • Molecular Biology
  • Cell Biology


Dive into the research topics of 'Hyaluronan stimulates chondrogenic gene expression in human meniscus cells'. Together they form a unique fingerprint.

Cite this