Hydrogen-rich saline protects against liver injury in rats with obstructive jaundice

Qu Liu, Wei Feng Shen, Han Yong Sun, Dan Feng Fan, Atsunori Nakao, Jian Mei Cai, Ge Yan, Wei Ping Zhou, Rong Xi Shen, Jia Mei Yang, Xue Jun Sun

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73 Citations (Scopus)


Background: Hydrogen selectively reduces levels of hydroxyl radicals and alleviates acute oxidative stress in many models. Hydrogen-rich saline provides a high concentration of hydrogen that can be easily and safely applied. Aims: In this study, we investigated the effects of hydrogen-rich saline on the prevention of liver injury induced by obstructive jaundice in rats. Methods: Male Sprague-Dawley rats (n=56) were divided randomly into four experimental groups: sham operated, bile duct ligation (BDL) plus saline treatment [5 ml/kg, intraperitoneal (i.p.)], BDL plus low-dose hydrogen-rich saline treatment (5 ml/kg, i.p.) and BDL plus high-dose hydrogen-rich saline treatment (10 ml/kg, i.p.). Results: The liver damage was evaluated microscopically 10 days after BDL. Serum alanine aminotransferase and aspartate aminotransferase levels, tissue malondialdehyde content, myeloperoxidase activity, tumour necrosis factor-α, interleukin (IL)-1β, IL-6 and high-mobility group box 1 levels were all increased significantly by BDL. Hydrogen-rich saline reduced levels of these markers and relieved morphological liver injury. Additionally, hydrogen-rich saline markedly increased the activities of anti-oxidant enzymes superoxide dismutase and catalase and downregulated extracellular signal-regulated protein kinase (ERK)1/2 activation. Conclusions: :Hydrogen-rich saline attenuates BDL-induced liver damage, possibly by the reduction of inflammation and oxidative stress and the inhibition of the ERK1/2 pathway.

Original languageEnglish
Pages (from-to)958-968
Number of pages11
JournalLiver International
Issue number7
Publication statusPublished - Aug 2010
Externally publishedYes


  • Anti-oxidant
  • Hydrogen-rich saline
  • Inflammation
  • Liver injury
  • Obstructive jaundice
  • Oxidative stress

ASJC Scopus subject areas

  • Hepatology


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