Hydrolysis of the oil phase of a W/O/W emulsion by pancreatic lipase

Motohiro Shima, Miyono Tanaka, Yukitaka Kimura, Shuji Adachi, Ryuichi Matsuno

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)


W/O/W emulsions are expected to protect bioactive substances from degradation by pancreatic enzymes. We investigated the enzymatic hydrolysis of the oil phase and release of a marker substance from the inner-aqueous phase to the outer-aqueous phase using an artificial digestive fluid. Octanoic acid triacylglycerol (C8TG) was used as the oil phase. W/O/W emulsions were prepared by two-step homogenization and succeeding membrane filtration. When the artificial digestive fluid containing lipase and gall was added to the emulsion, release of the marker substance from the inner-phase solution, oil-phase hydrolysis, and emulsion coalescence occurred in that order. When a coarse emulsion and 0.2- and 0.8-μm membrane-filtered fine emulsions were treated with the fluid for 1 h, the degrees of C8TG hydrolysis were 3.8%, 55% and 57%, the fractions of the marker substance released from the inner-water phase were 2.7%, 89% and 72%, and the median diameters of the oil droplets were changed from 32 to 23 μm, 0.71 to 27 μm, and from 2.2 to 26 μm, respectively. These results suggested that the diameter of the oil droplets in the W/O/W emulsion significantly affected the release profile of the marker loaded in the inner-water phase of the emulsion.

Original languageEnglish
Pages (from-to)53-61
Number of pages9
JournalJournal of Controlled Release
Issue number1
Publication statusPublished - Jan 8 2004
Externally publishedYes


  • Drug delivery
  • Hydrophilic bioactive substance
  • Lipid hydrolysis
  • Pancreatic lipase
  • W/O/W emulsion

ASJC Scopus subject areas

  • Pharmaceutical Science


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