Hydrothermal synthesis of porous hydroxyapatite plates from gypsum: Use as an animal cell culture substratum

Hiroyuki Ijima; Motonori Nakashima; Sachiko Furuta; Tsutomu Ono; Koei Kawakami

doi:10.1252/jcej.37.531

Hydrothermal synthesis of porous hydroxyapatite plates from gypsum: Use as an animal cell culture substratum

Hiroyuki Ijima, Motonori Nakashima, Sachiko Furuta, Tsutomu Ono, Koei Kawakami

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

A macroporous HAp culture substratum was formed by hydrothermal treatment of gypsum. The macropores derived from poly(methylmetacrylate) (PMMA) beads had diameters of 150-200 μm, and a porosity of 28% that was dependent on the PMMA beads quality. The maximum CHO-K1 cell density of the macroporous HAp microcarrier was 7.3 × 10⁷ cells·m/^-1-HAp. This value is similar to most existing suitable porous microcarriers. A macroporous HAp plate packed-bed type bioreactor was designed. The density of CHO-K1 cells in the bioreactor was seven times higher than that from a stationary culture. These results suggest that the HAp synthesized from gypsum by hydrothermal treatment is a suitable animal cell culture substratum.

Original language	English
Pages (from-to)	531-535
Number of pages	5
Journal	JOURNAL OF CHEMICAL ENGINEERING OF JAPAN
Volume	37
Issue number	4
DOIs	https://doi.org/10.1252/jcej.37.531
Publication status	Published - Apr 1 2004
Externally published	Yes

Keywords

CHO-K1 cells
Culture substratum
Gypsum
Hydrothermal treatment
Hydroxyapatite
Proliferation

ASJC Scopus subject areas

Chemistry(all)
Chemical Engineering(all)

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title = "Hydrothermal synthesis of porous hydroxyapatite plates from gypsum: Use as an animal cell culture substratum",

abstract = "A macroporous HAp culture substratum was formed by hydrothermal treatment of gypsum. The macropores derived from poly(methylmetacrylate) (PMMA) beads had diameters of 150-200 μm, and a porosity of 28% that was dependent on the PMMA beads quality. The maximum CHO-K1 cell density of the macroporous HAp microcarrier was 7.3 × 107 cells·m/-1-HAp. This value is similar to most existing suitable porous microcarriers. A macroporous HAp plate packed-bed type bioreactor was designed. The density of CHO-K1 cells in the bioreactor was seven times higher than that from a stationary culture. These results suggest that the HAp synthesized from gypsum by hydrothermal treatment is a suitable animal cell culture substratum.",

keywords = "CHO-K1 cells, Culture substratum, Gypsum, Hydrothermal treatment, Hydroxyapatite, Proliferation",

author = "Hiroyuki Ijima and Motonori Nakashima and Sachiko Furuta and Tsutomu Ono and Koei Kawakami",

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T2 - Use as an animal cell culture substratum

AU - Ijima, Hiroyuki

AU - Nakashima, Motonori

AU - Furuta, Sachiko

AU - Ono, Tsutomu

AU - Kawakami, Koei

PY - 2004/4/1

Y1 - 2004/4/1

N2 - A macroporous HAp culture substratum was formed by hydrothermal treatment of gypsum. The macropores derived from poly(methylmetacrylate) (PMMA) beads had diameters of 150-200 μm, and a porosity of 28% that was dependent on the PMMA beads quality. The maximum CHO-K1 cell density of the macroporous HAp microcarrier was 7.3 × 107 cells·m/-1-HAp. This value is similar to most existing suitable porous microcarriers. A macroporous HAp plate packed-bed type bioreactor was designed. The density of CHO-K1 cells in the bioreactor was seven times higher than that from a stationary culture. These results suggest that the HAp synthesized from gypsum by hydrothermal treatment is a suitable animal cell culture substratum.

AB - A macroporous HAp culture substratum was formed by hydrothermal treatment of gypsum. The macropores derived from poly(methylmetacrylate) (PMMA) beads had diameters of 150-200 μm, and a porosity of 28% that was dependent on the PMMA beads quality. The maximum CHO-K1 cell density of the macroporous HAp microcarrier was 7.3 × 107 cells·m/-1-HAp. This value is similar to most existing suitable porous microcarriers. A macroporous HAp plate packed-bed type bioreactor was designed. The density of CHO-K1 cells in the bioreactor was seven times higher than that from a stationary culture. These results suggest that the HAp synthesized from gypsum by hydrothermal treatment is a suitable animal cell culture substratum.

KW - CHO-K1 cells

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KW - Gypsum

KW - Hydrothermal treatment

KW - Hydroxyapatite

KW - Proliferation

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Hydrothermal synthesis of porous hydroxyapatite plates from gypsum: Use as an animal cell culture substratum

Abstract

Keywords

ASJC Scopus subject areas

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