TY - JOUR
T1 - Hyperlipidemia is involved in apoptosis in rat submandibular glands
AU - Irie, Koichiro
AU - Tomofuji, Takaaki
AU - Ekuni, Daisuke
AU - Azuma, Tetsuji
AU - Yoneda, Toshiki
AU - Shimazaki, Yoshihiro
AU - Morita, Manabu
N1 - Funding Information:
This work was supported by the Uehara Memorial Foundation, Tokyo, Japan and by Grants-in-Aid for Scientific Research (23390480 & 23792512) from the Ministry of Education, Culture, Sports, Science and Technology, Tokyo, Japan.
Publisher Copyright:
© 2017 Elsevier Ltd
PY - 2017/9
Y1 - 2017/9
N2 - Objective The aim of the present study was to investigate the effects of hyperlipidemia on histological changes and apoptosis in submandibular glands using apolipoprotein E (apoE)-deficient rats. Design Histopathological findings related to induced apoptosis in the submandibular glands were compared between apoE-deficient rats (n = 6; male; age, 16 weeks) and the corresponding wild-type rats (n = 6). Results ApoE-deficient rats showed significantly higher plasma levels of oxidized low-density lipoprotein (LDL), total cholesterol, very LDL and LDL, and lower plasma levels of high-density lipoprotein when compared to control rats (P < 0.05). Lipid deposition in the submandibular gland was observed in apoE-deficient rat group and in none of the control group. Significant increases in vacuolization and apoptosis in acinar cells were observed in apoE-deficient rats, as compared to control rats (P < 0.05). The number of active caspase-3-positive cells was also higher in the apoE-deficient rat group when compared with the control group (P < 0.01). Conclusions According to our results, hyperlipidemia induced apoptosis in apoE-deficient rat submandibular glands. Oxidized LDL generation in case of hyperlipidemia may trigger off a reaction of apoptotic acinar cells with vacuolization in the submandibular glands.
AB - Objective The aim of the present study was to investigate the effects of hyperlipidemia on histological changes and apoptosis in submandibular glands using apolipoprotein E (apoE)-deficient rats. Design Histopathological findings related to induced apoptosis in the submandibular glands were compared between apoE-deficient rats (n = 6; male; age, 16 weeks) and the corresponding wild-type rats (n = 6). Results ApoE-deficient rats showed significantly higher plasma levels of oxidized low-density lipoprotein (LDL), total cholesterol, very LDL and LDL, and lower plasma levels of high-density lipoprotein when compared to control rats (P < 0.05). Lipid deposition in the submandibular gland was observed in apoE-deficient rat group and in none of the control group. Significant increases in vacuolization and apoptosis in acinar cells were observed in apoE-deficient rats, as compared to control rats (P < 0.05). The number of active caspase-3-positive cells was also higher in the apoE-deficient rat group when compared with the control group (P < 0.01). Conclusions According to our results, hyperlipidemia induced apoptosis in apoE-deficient rat submandibular glands. Oxidized LDL generation in case of hyperlipidemia may trigger off a reaction of apoptotic acinar cells with vacuolization in the submandibular glands.
KW - Apoptosis
KW - Hyperlipidemia
KW - Rats
KW - Submandibular gland
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U2 - 10.1016/j.archoralbio.2017.05.004
DO - 10.1016/j.archoralbio.2017.05.004
M3 - Article
C2 - 28528308
AN - SCOPUS:85019461433
SN - 0003-9969
VL - 81
SP - 136
EP - 140
JO - Archives of Oral Biology
JF - Archives of Oral Biology
ER -