TY - JOUR
T1 - Hypoxia increases glucose transporter 1 expression in bovine corpus luteum at the early luteal stage
AU - Nishimura, Ryo
AU - Hasegawa, Hiroki
AU - Yamashita, Masamichi
AU - Ito, Norihiko
AU - Okamoto, Yoshiharu
AU - Takeuchi, Takashi
AU - Kubo, Tomoaki
AU - Iga, Kosuke
AU - Kimura, Koji
AU - Hishinuma, Mitsugu
AU - Okuda, Kiyoshi
N1 - Funding Information:
ACKNOWLEDGMENTS. This research was supported by Grant-in-Aid for Young Scientists (B) (Grant Number JP16K18803) of the Japan Society for the Promotion of Science (JSPS). We are grateful to Dr. Tsuyoshi Yamaguchi and Dr. Tatsufumi Usui of Tottori University for the technical assistance in measuring P4 concentrations. We are also grateful to Dr. Naoki Kitamura of Tottori University for the technical assistance in isolating bovine luteal cells.
Publisher Copyright:
© 2017 The Japanese Society of Veterinary Science.
PY - 2017/11
Y1 - 2017/11
N2 - A major role of the corpus luteum (CL) is to produce progesterone (P4). The CL has immature vasculature shortly after ovulation, suggesting it exists under hypoxic conditions. Hypoxia-inducible factor-1 (HIF1) induces the expression of glucose transporter 1 (GLUT1). To clarify the physiological roles of GLUT1 in bovine CL, we examined GLUT1 mRNA expression in the CL under hypoxic conditions by quantitative RT-PCR. We also measured the effects of glucose (0-25 mM) and GLUT1 inhibitors (cytochalasin B, STF-31) on P4 production in bovine luteal cells. GLUT1 mRNA expression in bovine CL was higher at the early luteal stage compared to the other later stages. Hypoxia (3% O2) increased GLUT1 mRNA expression in early luteal cells, but not in mid luteal cells. Glucose (0-25 mM) increased P4 production in early luteal cells, but not in mid luteal cells. Both GLUT1 inhibitors decreased P4 production in early and mid luteal cells. Overall, the results suggest that GLUT1 (possibly induced by hypoxic conditions in the early CL) plays a role in the establishment and development of bovine CL, especially in supporting luteal P4 synthesis at the early luteal stage.
AB - A major role of the corpus luteum (CL) is to produce progesterone (P4). The CL has immature vasculature shortly after ovulation, suggesting it exists under hypoxic conditions. Hypoxia-inducible factor-1 (HIF1) induces the expression of glucose transporter 1 (GLUT1). To clarify the physiological roles of GLUT1 in bovine CL, we examined GLUT1 mRNA expression in the CL under hypoxic conditions by quantitative RT-PCR. We also measured the effects of glucose (0-25 mM) and GLUT1 inhibitors (cytochalasin B, STF-31) on P4 production in bovine luteal cells. GLUT1 mRNA expression in bovine CL was higher at the early luteal stage compared to the other later stages. Hypoxia (3% O2) increased GLUT1 mRNA expression in early luteal cells, but not in mid luteal cells. Glucose (0-25 mM) increased P4 production in early luteal cells, but not in mid luteal cells. Both GLUT1 inhibitors decreased P4 production in early and mid luteal cells. Overall, the results suggest that GLUT1 (possibly induced by hypoxic conditions in the early CL) plays a role in the establishment and development of bovine CL, especially in supporting luteal P4 synthesis at the early luteal stage.
KW - Corpus luteum
KW - Glucose transporter-1
KW - HIF1
KW - Hypoxia
KW - Progesterone
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U2 - 10.1292/jvms.17-0284
DO - 10.1292/jvms.17-0284
M3 - Article
C2 - 29046497
AN - SCOPUS:85034633654
SN - 0916-7250
VL - 79
SP - 1878
EP - 1883
JO - Journal of Veterinary Medical Science
JF - Journal of Veterinary Medical Science
IS - 11
ER -