Identification and Characterization of Heat‐Stable Enterotoxin II‐Producing Escherichia coli from Patients with Diarrhea

Keinosuke Okamoto; Yoshio Fujii; Noriko Akashi; Shunji Hitotsubashi; Hisao Kurazono; Tadahiro Karasawa; Yoshifumi Takeda

doi:10.1111/j.1348-0421.1993.tb03230.x

Identification and Characterization of Heat‐Stable Enterotoxin II‐Producing Escherichia coli from Patients with Diarrhea

Keinosuke Okamoto, Yoshio Fujii, Noriko Akashi, Shunji Hitotsubashi, Hisao Kurazono, Tadahiro Karasawa, Yoshifumi Takeda

Research output: Contribution to journal › Article › peer-review

29 Citations (Scopus)

Abstract

Stock strains of Eschericia coli isolated from patients with traveller's diarrhea were examined for production of heat‐stable enterotoxin II (STII). Of 400 strains examined, 3 were found to produce STII. The nucleotide sequence of the STII gene of these human strains was shown to be identical to that of porcine strains. Cultured cells of these strains induced fluid accumulation in ligated mouse intestinal loops and the activity was neutralized by anti‐STII antiserum. These results suggest that STII‐produciing enterotoxigenic E. coli can cause human diarrhea.

Original language	English
Pages (from-to)	411-414
Number of pages	4
Journal	MICROBIOLOGY and IMMUNOLOGY
Volume	37
Issue number	5
DOIs	https://doi.org/10.1111/j.1348-0421.1993.tb03230.x
Publication status	Published - May 1993
Externally published	Yes

Keywords

diarrhea
escherichia coli
heat‐stable enterotoxin

ASJC Scopus subject areas

Microbiology
Immunology
Virology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1111/j.1348-0421.1993.tb03230.x

Cite this

@article{779e5be7172449038f49c254695da595,

title = "Identification and Characterization of Heat‐Stable Enterotoxin II‐Producing Escherichia coli from Patients with Diarrhea",

abstract = "Stock strains of Eschericia coli isolated from patients with traveller's diarrhea were examined for production of heat‐stable enterotoxin II (STII). Of 400 strains examined, 3 were found to produce STII. The nucleotide sequence of the STII gene of these human strains was shown to be identical to that of porcine strains. Cultured cells of these strains induced fluid accumulation in ligated mouse intestinal loops and the activity was neutralized by anti‐STII antiserum. These results suggest that STII‐produciing enterotoxigenic E. coli can cause human diarrhea.",

keywords = "diarrhea, escherichia coli, heat‐stable enterotoxin",

author = "Keinosuke Okamoto and Yoshio Fujii and Noriko Akashi and Shunji Hitotsubashi and Hisao Kurazono and Tadahiro Karasawa and Yoshifumi Takeda",

year = "1993",

month = may,

doi = "10.1111/j.1348-0421.1993.tb03230.x",

language = "English",

volume = "37",

pages = "411--414",

journal = "MICROBIOLOGY and IMMUNOLOGY",

issn = "0385-5600",

publisher = "Center for Academic Publications Japan",

number = "5",

}

TY - JOUR

T1 - Identification and Characterization of Heat‐Stable Enterotoxin II‐Producing Escherichia coli from Patients with Diarrhea

AU - Okamoto, Keinosuke

AU - Fujii, Yoshio

AU - Akashi, Noriko

AU - Hitotsubashi, Shunji

AU - Kurazono, Hisao

AU - Karasawa, Tadahiro

AU - Takeda, Yoshifumi

PY - 1993/5

Y1 - 1993/5

N2 - Stock strains of Eschericia coli isolated from patients with traveller's diarrhea were examined for production of heat‐stable enterotoxin II (STII). Of 400 strains examined, 3 were found to produce STII. The nucleotide sequence of the STII gene of these human strains was shown to be identical to that of porcine strains. Cultured cells of these strains induced fluid accumulation in ligated mouse intestinal loops and the activity was neutralized by anti‐STII antiserum. These results suggest that STII‐produciing enterotoxigenic E. coli can cause human diarrhea.

AB - Stock strains of Eschericia coli isolated from patients with traveller's diarrhea were examined for production of heat‐stable enterotoxin II (STII). Of 400 strains examined, 3 were found to produce STII. The nucleotide sequence of the STII gene of these human strains was shown to be identical to that of porcine strains. Cultured cells of these strains induced fluid accumulation in ligated mouse intestinal loops and the activity was neutralized by anti‐STII antiserum. These results suggest that STII‐produciing enterotoxigenic E. coli can cause human diarrhea.

KW - diarrhea

KW - escherichia coli

KW - heat‐stable enterotoxin

UR - http://www.scopus.com/inward/record.url?scp=0027201566&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027201566&partnerID=8YFLogxK

U2 - 10.1111/j.1348-0421.1993.tb03230.x

DO - 10.1111/j.1348-0421.1993.tb03230.x

M3 - Article

C2 - 8355624

AN - SCOPUS:0027201566

SN - 0385-5600

VL - 37

SP - 411

EP - 414

JO - MICROBIOLOGY and IMMUNOLOGY

JF - MICROBIOLOGY and IMMUNOLOGY

IS - 5

ER -

Identification and Characterization of Heat‐Stable Enterotoxin II‐Producing Escherichia coli from Patients with Diarrhea

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this