Identification and characterization of the Aeromonas sobria hemolysin glycoprotein receptor on Intestine 407 cells

Ai Ping Wang; Akihiro Wada; Kinnosuke Yahiro; Tomohiko Nomura; Yoshio Fujii; Keinoske Okamoto; Youhei Mizuta; Shigeru Kohno; Joel Moss; Toshiya Hirayama

doi:10.1006/mpat.1999.0299

Identification and characterization of the Aeromonas sobria hemolysin glycoprotein receptor on Intestine 407 cells

Ai Ping Wang, Akihiro Wada, Kinnosuke Yahiro, Tomohiko Nomura, Yoshio Fujii, Keinoske Okamoto, Youhei Mizuta, Shigeru Kohno, Joel Moss, Toshiya Hirayama

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

Aeromonas sobria hemolysin is important in the pathogenesis of diarrhoea caused by this enteropathogenic bacterium. By immunoprecipitation analysis using hemolysin and anti-hemolysin antibody, a 66 kDa protein (p66) was identified as a receptor for A. sobria hemolysin on Intestine 407 cells. Treatment of p66 with N-glycosidase F reduced the apparent sized of p66 to 60 kDa on SDS-polyacrylamide gels. p66, released from Intestine 407 cells following incubation with phosphatidylinositol-specific phospholipase C (PI-PLC) treatment, bound A. sobria hemolysin. Thus treatment of Intestine 407 cells with PI-PLC resulted in the remarkable decrease of the sensitivity to A. sobria hemolysin. These results are consistent with the hypothesis that p66, the binding protein for A. sobria hemolysin, is a glycosylphosphatidylinositol-anchored glycoprotein expressed on the surface of Intestine 407 cells and probably plays a role as a receptor for A. sobria hemolysin on the intestinal cells.

Original language	English
Pages (from-to)	215-221
Number of pages	7
Journal	Microbial Pathogenesis
Volume	27
Issue number	4
DOIs	https://doi.org/10.1006/mpat.1999.0299
Publication status	Published - Oct 1999
Externally published	Yes

Keywords

Aeromonas sobria
Glycoprotein receptor
Hemolysin
Toxin action
Toxin receptor

ASJC Scopus subject areas

Microbiology
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1006/mpat.1999.0299

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title = "Identification and characterization of the Aeromonas sobria hemolysin glycoprotein receptor on Intestine 407 cells",

abstract = "Aeromonas sobria hemolysin is important in the pathogenesis of diarrhoea caused by this enteropathogenic bacterium. By immunoprecipitation analysis using hemolysin and anti-hemolysin antibody, a 66 kDa protein (p66) was identified as a receptor for A. sobria hemolysin on Intestine 407 cells. Treatment of p66 with N-glycosidase F reduced the apparent sized of p66 to 60 kDa on SDS-polyacrylamide gels. p66, released from Intestine 407 cells following incubation with phosphatidylinositol-specific phospholipase C (PI-PLC) treatment, bound A. sobria hemolysin. Thus treatment of Intestine 407 cells with PI-PLC resulted in the remarkable decrease of the sensitivity to A. sobria hemolysin. These results are consistent with the hypothesis that p66, the binding protein for A. sobria hemolysin, is a glycosylphosphatidylinositol-anchored glycoprotein expressed on the surface of Intestine 407 cells and probably plays a role as a receptor for A. sobria hemolysin on the intestinal cells.",

keywords = "Aeromonas sobria, Glycoprotein receptor, Hemolysin, Toxin action, Toxin receptor",

author = "Wang, {Ai Ping} and Akihiro Wada and Kinnosuke Yahiro and Tomohiko Nomura and Yoshio Fujii and Keinoske Okamoto and Youhei Mizuta and Shigeru Kohno and Joel Moss and Toshiya Hirayama",

note = "Funding Information: This work was supported by grants in aid from the Ministry of Education, Science and Culture of Japan and from Sasakawa Health Science Foundation.",

year = "1999",

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doi = "10.1006/mpat.1999.0299",

language = "English",

volume = "27",

pages = "215--221",

journal = "Microbial Pathogenesis",

issn = "0882-4010",

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TY - JOUR

T1 - Identification and characterization of the Aeromonas sobria hemolysin glycoprotein receptor on Intestine 407 cells

AU - Wang, Ai Ping

AU - Wada, Akihiro

AU - Yahiro, Kinnosuke

AU - Nomura, Tomohiko

AU - Fujii, Yoshio

AU - Okamoto, Keinoske

AU - Mizuta, Youhei

AU - Kohno, Shigeru

AU - Moss, Joel

AU - Hirayama, Toshiya

N1 - Funding Information: This work was supported by grants in aid from the Ministry of Education, Science and Culture of Japan and from Sasakawa Health Science Foundation.

PY - 1999/10

Y1 - 1999/10

N2 - Aeromonas sobria hemolysin is important in the pathogenesis of diarrhoea caused by this enteropathogenic bacterium. By immunoprecipitation analysis using hemolysin and anti-hemolysin antibody, a 66 kDa protein (p66) was identified as a receptor for A. sobria hemolysin on Intestine 407 cells. Treatment of p66 with N-glycosidase F reduced the apparent sized of p66 to 60 kDa on SDS-polyacrylamide gels. p66, released from Intestine 407 cells following incubation with phosphatidylinositol-specific phospholipase C (PI-PLC) treatment, bound A. sobria hemolysin. Thus treatment of Intestine 407 cells with PI-PLC resulted in the remarkable decrease of the sensitivity to A. sobria hemolysin. These results are consistent with the hypothesis that p66, the binding protein for A. sobria hemolysin, is a glycosylphosphatidylinositol-anchored glycoprotein expressed on the surface of Intestine 407 cells and probably plays a role as a receptor for A. sobria hemolysin on the intestinal cells.

AB - Aeromonas sobria hemolysin is important in the pathogenesis of diarrhoea caused by this enteropathogenic bacterium. By immunoprecipitation analysis using hemolysin and anti-hemolysin antibody, a 66 kDa protein (p66) was identified as a receptor for A. sobria hemolysin on Intestine 407 cells. Treatment of p66 with N-glycosidase F reduced the apparent sized of p66 to 60 kDa on SDS-polyacrylamide gels. p66, released from Intestine 407 cells following incubation with phosphatidylinositol-specific phospholipase C (PI-PLC) treatment, bound A. sobria hemolysin. Thus treatment of Intestine 407 cells with PI-PLC resulted in the remarkable decrease of the sensitivity to A. sobria hemolysin. These results are consistent with the hypothesis that p66, the binding protein for A. sobria hemolysin, is a glycosylphosphatidylinositol-anchored glycoprotein expressed on the surface of Intestine 407 cells and probably plays a role as a receptor for A. sobria hemolysin on the intestinal cells.

KW - Aeromonas sobria

KW - Glycoprotein receptor

KW - Hemolysin

KW - Toxin action

KW - Toxin receptor

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Identification and characterization of the Aeromonas sobria hemolysin glycoprotein receptor on Intestine 407 cells

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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