Identification and determination of 8α9αepoxyhexahydrocannabinol as a metabolite of δ8-tetrahydrocannabinol of mice in vitro

S. Narimatsu; K. Watanabe; I. Yamamoto; H. Yoshimura

doi:10.3109/00498258209038935

Identification and determination of 8α9αepoxyhexahydrocannabinol as a metabolite of δ⁸-tetrahydrocannabinol of mice in vitro

S. Narimatsu, K. Watanabe, I. Yamamoto, H. Yoshimura

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

1. 8α9αEpoxyhexahydrocannabinol (8α9αEHHC) was formed from δ8-tetrahydro-cannabinol (δ8-THC) by mouse liver microsomal preparation in the presence of an Nadph-generating system. 2. The epoxide was identified by t.l.c., g.l.c. and g.l.c.-mass spectrometry and, together with 11-hydroxy-δ8-THC (11-OH-δ⁸-THC), was determined by g.l.c. 3. When δ⁸-THC was incubated with mouse liver microsomal preparation, 8α9αEHHC and 11-OH-δ⁸-THC was formed to the extents of 14% and 23% respectively, of the added δ⁸-THC.

Original language	English
Pages (from-to)	561-565
Number of pages	5
Journal	Xenobiotica
Volume	12
Issue number	9
DOIs	https://doi.org/10.3109/00498258209038935
Publication status	Published - 1982
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Toxicology
Pharmacology
Health, Toxicology and Mutagenesis

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10.3109/00498258209038935

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title = "Identification and determination of 8α9αepoxyhexahydrocannabinol as a metabolite of δ8-tetrahydrocannabinol of mice in vitro",

abstract = "1. 8α9αEpoxyhexahydrocannabinol (8α9αEHHC) was formed from δ8-tetrahydro-cannabinol (δ8-THC) by mouse liver microsomal preparation in the presence of an Nadph-generating system. 2. The epoxide was identified by t.l.c., g.l.c. and g.l.c.-mass spectrometry and, together with 11-hydroxy-δ8-THC (11-OH-δ8-THC), was determined by g.l.c. 3. When δ8-THC was incubated with mouse liver microsomal preparation, 8α9αEHHC and 11-OH-δ8-THC was formed to the extents of 14% and 23% respectively, of the added δ8-THC.",

author = "S. Narimatsu and K. Watanabe and I. Yamamoto and H. Yoshimura",

year = "1982",

doi = "10.3109/00498258209038935",

language = "English",

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journal = "Xenobiotica",

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T1 - Identification and determination of 8α9αepoxyhexahydrocannabinol as a metabolite of δ8-tetrahydrocannabinol of mice in vitro

AU - Narimatsu, S.

AU - Watanabe, K.

AU - Yamamoto, I.

AU - Yoshimura, H.

PY - 1982

Y1 - 1982

N2 - 1. 8α9αEpoxyhexahydrocannabinol (8α9αEHHC) was formed from δ8-tetrahydro-cannabinol (δ8-THC) by mouse liver microsomal preparation in the presence of an Nadph-generating system. 2. The epoxide was identified by t.l.c., g.l.c. and g.l.c.-mass spectrometry and, together with 11-hydroxy-δ8-THC (11-OH-δ8-THC), was determined by g.l.c. 3. When δ8-THC was incubated with mouse liver microsomal preparation, 8α9αEHHC and 11-OH-δ8-THC was formed to the extents of 14% and 23% respectively, of the added δ8-THC.

AB - 1. 8α9αEpoxyhexahydrocannabinol (8α9αEHHC) was formed from δ8-tetrahydro-cannabinol (δ8-THC) by mouse liver microsomal preparation in the presence of an Nadph-generating system. 2. The epoxide was identified by t.l.c., g.l.c. and g.l.c.-mass spectrometry and, together with 11-hydroxy-δ8-THC (11-OH-δ8-THC), was determined by g.l.c. 3. When δ8-THC was incubated with mouse liver microsomal preparation, 8α9αEHHC and 11-OH-δ8-THC was formed to the extents of 14% and 23% respectively, of the added δ8-THC.

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Identification and determination of 8α9αepoxyhexahydrocannabinol as a metabolite of δ⁸-tetrahydrocannabinol of mice in vitro

Abstract

ASJC Scopus subject areas

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