TY - JOUR
T1 - Identification and molecular characterization of Porphyromonas gulae fimA types among cat isolates
AU - Iwashita, Naoki
AU - Nomura, Ryota
AU - Shirai, Mitsuyuki
AU - Kato, Yukio
AU - Murakami, Masaru
AU - Matayoshi, Saaya
AU - Kadota, Tamami
AU - Shirahata, So
AU - Ohzeki, Leo
AU - Arai, Nobuaki
AU - Yasuda, Junya
AU - Yasuda, Hidemi
AU - Inaba, Hiroaki
AU - Matsumoto-Nakano, Michiyo
AU - Nakano, Kazuhiko
AU - Asai, Fumitoshi
N1 - Funding Information:
The authors thank Prof. Howard K. Kuramitsu (State University of New York at Buffalo) for editing the manuscript. We also thank Ms. Rewa Yanagisawa, WDB Co., Ltd., for technical support with the molecular analyses. This study was supported by the Promotion and Mutual Aid Corporation for Private Schools of Japan (PMAC; No. 1729). All authors read and approved the final manuscript.
Publisher Copyright:
© 2018 Elsevier B.V.
PY - 2019/2
Y1 - 2019/2
N2 - Porphyromonas gulae, a Gram-negative black-pigmented anaerobe, is one of several major periodontal pathogens of animals. P. gulae isolates from dogs have been classified into three genotypes based on a 41-kDa filamentous appendage (FimA) on the cell surface, which is closely related to virulence in periodontal disease. However, other specific bacterial virulence factors contributing to the aggravation of periodontal disease in cats remain elusive. In the present study, we assessed FimA diversity in P. gulae isolates from cats and examined whether this diversity influenced periodontal condition. The putative amino acid sequences of FimA from 15 P. gulae isolates from 13 cats were classified into three genotypes (types A, B, and C), which showed 95–100% identity and similarity to the fimA types in dogs. The type C isolate showed greater adhesion and invasion properties in periodontal ligament fibroblasts as well as stronger inhibition of scratch closure of the cells compared with type A and B isolates. Next, a PCR-based method for identification of fimA genotype was developed and used to analyze 99 oral swab specimens from cats. High fimA type A detection rates were observed regardless of the periodontal condition, whereas types B and C were frequently detected from subjects with moderate and severe periodontitis, respectively. These results suggest that P. gulae isolates from cats can be classified into three types based on fimA genotype, which may be closely related to virulence in periodontitis.
AB - Porphyromonas gulae, a Gram-negative black-pigmented anaerobe, is one of several major periodontal pathogens of animals. P. gulae isolates from dogs have been classified into three genotypes based on a 41-kDa filamentous appendage (FimA) on the cell surface, which is closely related to virulence in periodontal disease. However, other specific bacterial virulence factors contributing to the aggravation of periodontal disease in cats remain elusive. In the present study, we assessed FimA diversity in P. gulae isolates from cats and examined whether this diversity influenced periodontal condition. The putative amino acid sequences of FimA from 15 P. gulae isolates from 13 cats were classified into three genotypes (types A, B, and C), which showed 95–100% identity and similarity to the fimA types in dogs. The type C isolate showed greater adhesion and invasion properties in periodontal ligament fibroblasts as well as stronger inhibition of scratch closure of the cells compared with type A and B isolates. Next, a PCR-based method for identification of fimA genotype was developed and used to analyze 99 oral swab specimens from cats. High fimA type A detection rates were observed regardless of the periodontal condition, whereas types B and C were frequently detected from subjects with moderate and severe periodontitis, respectively. These results suggest that P. gulae isolates from cats can be classified into three types based on fimA genotype, which may be closely related to virulence in periodontitis.
KW - Cat
KW - FimA
KW - Oral swab specimens
KW - Periodontal condition
KW - Periodontal ligament fibroblasts
KW - Porphyromonas gulae
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U2 - 10.1016/j.vetmic.2018.12.018
DO - 10.1016/j.vetmic.2018.12.018
M3 - Article
C2 - 30642584
AN - SCOPUS:85059121410
SN - 0378-1135
VL - 229
SP - 100
EP - 109
JO - Veterinary Microbiology
JF - Veterinary Microbiology
ER -