Identification of an HLA-A24-restricted OY-TES-1 epitope recognized by cytotoxic T-cells

Hideo Okumura, Yuji Noguchi, Akiko Uenaka, Toshiki Aji, Toshiro Ono, Kazuhiko Nakagawa, Motoi Aoe, Nobuyoshi Shimizu, Eiichi Nakayama

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)


OY-TES-1 was identified as a human homologue of the mouse, guinea pig, and pig proacrosin binding protein sp32 precursor. Differential expression levels of OY-TES-1 mRNA between testis and other normal tissues, and its expression in cancers indicated that OY-TES-1 would be classified as a cancer/testis antigen and considered to be a candidate of target antigen for cancer immunotherapy. In this study, we showed identification of HLA-A24-binding OY-TES-1 peptide, TES401-409, (KTPFVSPLL) recognized by CD8 T-cells. Purified CD8 T-cells from healthy donors stimulated in vitro with the peptide-pulsed autologous DC and PBMC produced IFNγ in response to the peptide-pulsed PBMC and showed cytotoxicity against the peptide-pulsed autologous EBV-B specifically. Furthermore, cytotoxicity was also observed against an OY-TES-1 mRNA-expressing tumor line, LK79. The retention time of the fraction in HPLC of the acid eluate from LK79 cells that showed positive sensitization against autologous EBV-B cells in recognition by CD8 CTL was the same as that of the fraction of the TES401-409 peptide itself, suggesting that the TES401-409 was a naturally processed peptide on LK79.

Original languageEnglish
Pages (from-to)1009-1016
Number of pages8
Issue number11
Publication statusPublished - 2005
Externally publishedYes


  • Acid extraction
  • CTL epitope
  • Cancer/testis antigen
  • OY-TES-1

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Virology


Dive into the research topics of 'Identification of an HLA-A24-restricted OY-TES-1 epitope recognized by cytotoxic T-cells'. Together they form a unique fingerprint.

Cite this