Identification of genes expressed during spore germination of Mycosphaerella pinodes

Hiroyuki Takahara; Kazuhiro Toyoda; Gento Tsuji; Yasuyuki Kubo; Yoshishige Inagaki; Yuki Ichinose; Tomonori Shiraishi

doi:10.1007/s10327-005-0185-4

Identification of genes expressed during spore germination of Mycosphaerella pinodes

Hiroyuki Takahara, Kazuhiro Toyoda, Gento Tsuji, Yasuyuki Kubo, Yoshishige Inagaki, Yuki Ichinose, Tomonori Shiraishi

School of Agriculture

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Mycosphaerella blight, caused by Mycosphaerella pinodes, is one of the major diseases of cultivated pea (Pisum sativum L.). To isolate the genes that are up- and down-regulated during spore germination, suppression subtraction hybridization (SSH) was performed between ungerminated and germinated spores. The 232 and 128 clones from forward and reverse libraries, respectively, were collected, sequenced, and analyzed with a BLASTX homology search. About 95% of the 32 selected clones were expressed during spore germination on a paper sheet and during infection of pea leaves. We discuss the applicability of the SSH libraries for analyzing M. pinodes genes involved in the early stage of infection.

Original language	English
Pages (from-to)	190-195
Number of pages	6
Journal	Journal of General Plant Pathology
Volume	71
Issue number	3
DOIs	https://doi.org/10.1007/s10327-005-0185-4
Publication status	Published - Jun 1 2005

Keywords

Mycosphaerella pinodes
Pisum sativum
Pycnospore germination
RT-PCR
Suppression subtractive hybridization (SSH)

ASJC Scopus subject areas

Agronomy and Crop Science
Plant Science

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10.1007/s10327-005-0185-4

Cite this

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abstract = "Mycosphaerella blight, caused by Mycosphaerella pinodes, is one of the major diseases of cultivated pea (Pisum sativum L.). To isolate the genes that are up- and down-regulated during spore germination, suppression subtraction hybridization (SSH) was performed between ungerminated and germinated spores. The 232 and 128 clones from forward and reverse libraries, respectively, were collected, sequenced, and analyzed with a BLASTX homology search. About 95% of the 32 selected clones were expressed during spore germination on a paper sheet and during infection of pea leaves. We discuss the applicability of the SSH libraries for analyzing M. pinodes genes involved in the early stage of infection.",

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AU - Takahara, Hiroyuki

AU - Toyoda, Kazuhiro

AU - Tsuji, Gento

AU - Kubo, Yasuyuki

AU - Inagaki, Yoshishige

AU - Ichinose, Yuki

AU - Shiraishi, Tomonori

PY - 2005/6/1

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AB - Mycosphaerella blight, caused by Mycosphaerella pinodes, is one of the major diseases of cultivated pea (Pisum sativum L.). To isolate the genes that are up- and down-regulated during spore germination, suppression subtraction hybridization (SSH) was performed between ungerminated and germinated spores. The 232 and 128 clones from forward and reverse libraries, respectively, were collected, sequenced, and analyzed with a BLASTX homology search. About 95% of the 32 selected clones were expressed during spore germination on a paper sheet and during infection of pea leaves. We discuss the applicability of the SSH libraries for analyzing M. pinodes genes involved in the early stage of infection.

KW - Mycosphaerella pinodes

KW - Pisum sativum

KW - Pycnospore germination

KW - RT-PCR

KW - Suppression subtractive hybridization (SSH)

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Identification of genes expressed during spore germination of Mycosphaerella pinodes

Abstract

Keywords

ASJC Scopus subject areas

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