Identification of osteopontin in isolated rabbit osteoclasts

Ken ichi Tezuka, Takuya Sato, Hiroshi Kamioka, Peter J. Nijweide, Kayo Tanaka, Tetsu Matsuo, Mitsue Ohta, Noriyoshi Kurihara, Yoshiyuki Hakeda, Masayoshi Kumegawa

Research output: Contribution to journalArticlepeer-review

203 Citations (Scopus)


Bone remodeling is a complex process coupling bone formation and resorption. Osteoblasts, the bone-forming cells, are known to produce various bone matrix proteins and cytokines; however, little is known about protein factors produced by osteoclasts or bone-resorbing cells. A method utilizing the high affinity of osteoclasts for tissue culture dishes was developed to isolate a large number of pure osteoclasts from rabbit long bones. A cDNA library was then constructed from these isolated osteoclasts, and differential cDNA screening was performed between osteoclasts and spleen cells. Two clones representing osteoclast-specific clones, named OC-1 and OC-2, were isolated. By Northern blot analysis, OC-1 was expressed in osteoclasts and in kidneys, whereas OC-2 was specific for osteoclasts. OC-1 was found to encode osteopontin from its nucleotide sequence, and therefore, osteopontin may have other functions for osteoclastic bone resorption besides osteoclast attachment to bone.

Original languageEnglish
Pages (from-to)911-917
Number of pages7
JournalBiochemical and Biophysical Research Communications
Issue number2
Publication statusPublished - Jul 31 1992
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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