Identification of proacrosin binding protein sp32 precursor as a human cancer/testis antigen

Toshiro Ono, Takushi Kurashige, Naoki Harada, Yuji Noguchi, Takashi Saika, Norio Niikawa, Motoi Aoe, Shinichiro Nakamura, Toshihiro Higashi, Akio Hiraki, Hisashi Wada, Hiromi Kumon, Lloyd J. Old, Eiichi Nakayama

Research output: Contribution to journalArticlepeer-review

41 Citations (Scopus)


Serological expression cloning of antigens eliciting a humoral immune response to a syngeneic mouse sarcoma identified pem (mouse placenta and embryonic expression gene) as a new member of the cancer/testis family. To identify the human homologue of pem, mouse pem sequences and pem-related expressed sequence tags from human testis were used as PCR primers for amplification using human testis cDNA. However, rather than pem, another gene, designated OY-TES-1, was isolated and found to be the human homologue of proacrosin binding protein sp32 precursor originally identified in mouse, guinea pig, and pig. OY-TES-1 maps to chromosome 12p12-p13 and contains 10 exons. Southern blot analysis suggests the presence of two OY-TES-1-related genes in the human genome. In normal tissues, OY-TES-1 mRNA was expressed only in testis, whereas in malignant tissues, a variable proportion of a wide array of cancers, including bladder, breast, lung, liver, and colon cancers, expressed OY-TES-1. Serological survey of 362 cancer patients with a range of different cancers showed antibody to OY-TES-1 in 25 patients. No OY-TES-1 sera reactivity was found in 20 normal individuals. These findings indicate that OY-TES-1 is an additional member of the cancer/testis family of antigens and that OY-TES-1 is immunogenic in humans.

Original languageEnglish
Pages (from-to)3282-3287
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number6
Publication statusPublished - Mar 13 2001
Externally publishedYes


  • Antibody response
  • PCR cloning
  • Testis cDNA

ASJC Scopus subject areas

  • General


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