TY - JOUR
T1 - Identification of qtls for rice grain size using a novel set of chromosomal segment substitution lines derived from yamadanishiki in the genetic background of koshihikari
AU - Okada, Satoshi
AU - Onogi, Akio
AU - Iijima, Ken
AU - Hori, Kiyosumi
AU - Iwata, Hiroyoshi
AU - Yokoyama, Wakana
AU - Suehiro, Miki
AU - Yamasaki, Masanori
N1 - Funding Information:
We thank Prof. Shigeo Takumi (Kobe University, Japan) for use of the epMotion 5070 pipetting machine. This work was supported by JSPS KAKENHI Grant Number 17J01082 and Council for Science, Technology and Innovation (CSTI), Cross-ministerial Strategic Innovation Promotion Program (SIP), “Technologies for creating next-generation agriculture, forestry and fisheries” (funding agency: Bio-oriented Technology Research Advancement Institute, NARO).
Publisher Copyright:
© 2018, Japanese Society of Breeding. All rights reserved.
PY - 2018
Y1 - 2018
N2 - Grain size is important for brewing-rice cultivars, but the genetic basis for this trait is still unclear. This paper aims to identify QTLs for grain size using novel chromosomal segment substitution lines (CSSLs) harboring chromosomal segments from Yamadanishiki, an excellent sake-brewing rice, in the genetic background of Koshihikari, a cooking cultivar. We developed a set of 49 CSSLs. Grain length (GL), grain width (GWh), grain thickness (GT), 100-grain weight (GWt) and days to heading (DTH) were evaluated, and a CSSL-QTL analysis was conducted. Eighteen QTLs for grain size and DTH were identified. Seven (qGL11, qGWh5, qGWh10, qGWt6-2, qGWt10-2, qDTH3, and qDTH6) that were detected in F2 and recombinant inbred lines (RILs) from Koshihikari/Yamadanishiki were validated, suggesting that they are important for large grain size and heading date in Yamadanishiki. Additionally, QTL reanalysis for GWt showed that qGWt10-2 was only detected in early-flowering RILs, while qGWt5 (in the same region as qGWh5) was only detected in late-flowering RILs, suggesting that these QTLs show different responses to the environment. Our study revealed that grain size in the Yamadanishiki cultivar is determined by a complex genetic mechanism. These findings could be useful for the breeding of both cooking and brewing rice.
AB - Grain size is important for brewing-rice cultivars, but the genetic basis for this trait is still unclear. This paper aims to identify QTLs for grain size using novel chromosomal segment substitution lines (CSSLs) harboring chromosomal segments from Yamadanishiki, an excellent sake-brewing rice, in the genetic background of Koshihikari, a cooking cultivar. We developed a set of 49 CSSLs. Grain length (GL), grain width (GWh), grain thickness (GT), 100-grain weight (GWt) and days to heading (DTH) were evaluated, and a CSSL-QTL analysis was conducted. Eighteen QTLs for grain size and DTH were identified. Seven (qGL11, qGWh5, qGWh10, qGWt6-2, qGWt10-2, qDTH3, and qDTH6) that were detected in F2 and recombinant inbred lines (RILs) from Koshihikari/Yamadanishiki were validated, suggesting that they are important for large grain size and heading date in Yamadanishiki. Additionally, QTL reanalysis for GWt showed that qGWt10-2 was only detected in early-flowering RILs, while qGWt5 (in the same region as qGWh5) was only detected in late-flowering RILs, suggesting that these QTLs show different responses to the environment. Our study revealed that grain size in the Yamadanishiki cultivar is determined by a complex genetic mechanism. These findings could be useful for the breeding of both cooking and brewing rice.
KW - Brewing-rice cultivar
KW - CSSLs
KW - Grain size
KW - QTL
KW - QTL-by-environment interaction
UR - http://www.scopus.com/inward/record.url?scp=85047493131&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85047493131&partnerID=8YFLogxK
U2 - 10.1270/jsbbs.17112
DO - 10.1270/jsbbs.17112
M3 - Article
AN - SCOPUS:85047493131
SN - 1344-7610
VL - 68
SP - 210
EP - 218
JO - Breeding Science
JF - Breeding Science
IS - 2
ER -