TY - JOUR
T1 - Identification of testis specific calcineurin β subunit isoform by a monoclonal antibody and detection of a specific six amino acid sequence
AU - Nishio, Hajime
AU - Matsui, Hideki
AU - Etoh, Seiji
AU - Moia, Lizomar J.M.P.
AU - Tokuda, Masaaki
AU - Itano, Toshifumi
AU - Hatase, Osamu
PY - 1992/1/15
Y1 - 1992/1/15
N2 - Two isoforms of calcineurin β subunit(β1 and β2) were identified in rat testis by a monoclonal antibody Val. Both β1 and β2 were recovered in calmodulin binding protein fraction and showed calcium shift on SDS-polyacrylamide gel electrophoresis which is the specific character for EF-hand calcium binding protein. β2 showed same apparent molecular weight on SDS-PAGE as that of brain calcineurin and was found in wide variety of tissues. β1 was shown to have six amino acid polypepeptide sequence and it showed higher molecular weight than brain β and was specific for testis.
AB - Two isoforms of calcineurin β subunit(β1 and β2) were identified in rat testis by a monoclonal antibody Val. Both β1 and β2 were recovered in calmodulin binding protein fraction and showed calcium shift on SDS-polyacrylamide gel electrophoresis which is the specific character for EF-hand calcium binding protein. β2 showed same apparent molecular weight on SDS-PAGE as that of brain calcineurin and was found in wide variety of tissues. β1 was shown to have six amino acid polypepeptide sequence and it showed higher molecular weight than brain β and was specific for testis.
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U2 - 10.1016/S0006-291X(05)80108-1
DO - 10.1016/S0006-291X(05)80108-1
M3 - Article
C2 - 1310015
AN - SCOPUS:0026584711
SN - 0006-291X
VL - 182
SP - 34
EP - 38
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -