TY - JOUR
T1 - IL-8 is involved in homologous TNFα-, but not in IL-1β-induced neutrophil infiltration in rabbits
AU - Miyamoto, K.
AU - Matsukawa, A.
AU - Ohkawara, S.
AU - Takagi, K.
AU - Yoshinaga, M.
N1 - Funding Information:
Acknowledgments. We thank M. Ohara for helpful comments. This work was supported in part by grants from The Ministry of Education, Science, Sports and Culture, and The Ministry of Health and Welfare, Japan.
PY - 1997
Y1 - 1997
N2 - Objective and Design: We examined the involvement of IL-8 in TNFα and IL-1β neutrophil infiltration, using homologous reagents. Subjects: Female New Zealand white rabbits (n = 211). Methods: After injection of TNFα (1 μg) or rrIL-1β (300 pg) into rabbit knee joints, the leukocyte influx and the production of IL-8 were estimated. Anti-TNFα mAb, rrIL-1Ra, and anti-IL-8 IgG (10 μg each) were administrated simultaneously with either TNFα or IL-1β. Results: Administration of TNFα or IL-1β induced IL-8 production; both of which peaked at 1 h after the injection. Maximum neutrophil infiltration induced by TNFα was inhibited by 38-56% by anti-IL-8 IgG, 22-43% by rrIL-1Ra, and 77-83% by anti-IL-8 IgG plus rrIL-1Ra. In contrast, maximum neutrophil infiltration induced by IL-1β was not inhibited by anti-IL-8 IgG whereas anti-TNFα mAb reduced the infiltration by 68-75%. Conclusions: The involvement of IL-8 in TNFα- and IL-1β-induced neutrophil infiltration is limited. Factor(s) other than IL-8 appear to function as major neutrophil chemoattractants.
AB - Objective and Design: We examined the involvement of IL-8 in TNFα and IL-1β neutrophil infiltration, using homologous reagents. Subjects: Female New Zealand white rabbits (n = 211). Methods: After injection of TNFα (1 μg) or rrIL-1β (300 pg) into rabbit knee joints, the leukocyte influx and the production of IL-8 were estimated. Anti-TNFα mAb, rrIL-1Ra, and anti-IL-8 IgG (10 μg each) were administrated simultaneously with either TNFα or IL-1β. Results: Administration of TNFα or IL-1β induced IL-8 production; both of which peaked at 1 h after the injection. Maximum neutrophil infiltration induced by TNFα was inhibited by 38-56% by anti-IL-8 IgG, 22-43% by rrIL-1Ra, and 77-83% by anti-IL-8 IgG plus rrIL-1Ra. In contrast, maximum neutrophil infiltration induced by IL-1β was not inhibited by anti-IL-8 IgG whereas anti-TNFα mAb reduced the infiltration by 68-75%. Conclusions: The involvement of IL-8 in TNFα- and IL-1β-induced neutrophil infiltration is limited. Factor(s) other than IL-8 appear to function as major neutrophil chemoattractants.
KW - IL-1 receptor antagonist
KW - IL-1β
KW - IL-8
KW - Neutrophil infiltration
KW - TNFα
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U2 - 10.1007/s000110050227
DO - 10.1007/s000110050227
M3 - Article
C2 - 9427069
AN - SCOPUS:0030725611
SN - 1023-3830
VL - 46
SP - 472
EP - 477
JO - Inflammation Research
JF - Inflammation Research
IS - 11
ER -