Imaging the efficacy of UVC irradiation on superficial brain tumors and metastasis in live mice at the subcellular level

Masashi Momiyama, Atsushi Suetsugu, Hiroaki Kimura, Hiroyuki Kishimoto, Ryoichi Aki, Akimitsu Yamada, Harumi Sakurada, Takashi Chishima, Michael Bouvet, Itaru Endo, Robert M. Hoffman

Research output: Contribution to journalArticlepeer-review

40 Citations (Scopus)


The effect of UVC irradiation was investigated on a model of brain cancer and a model of experimental brain metastasis. For the brain cancer model, brain cancer cells were injected stereotactically into the brain. For the brain metastasis model, lung cancer cells were injected intracarotidally or stereotactically. The U87 human glioma cell line was used for the brain cancer model, and the Lewis lung carcinoma (LLC) was used for the experimental brain metastasis model. Both cancer cell types were labeled with GFP in the nucleus and RFP in the cytoplasm. A craniotomy open window was used to image single cancer cells in the brain. This double labeling of the cancer cells with GFP and RFP enabled apoptosis of single cells to be imaged at the subcellular level through the craniotomy open window. UVC irradiation, beamed through the craniotomy open window, induced apoptosis in the cancer cells. UVC irradiation was effective on LLC and significantly extended survival of the mice with experimental brain metastasis. In contrast, the U87 glioma was relatively resistant to UVC irradiation. The results of this study suggest the use of UVC for treatment of superficial brain cancer or metastasis.

Original languageEnglish
Pages (from-to)428-434
Number of pages7
JournalJournal of Cellular Biochemistry
Issue number2
Publication statusPublished - Feb 2013
Externally publishedYes


  • Apoptosis
  • Brain cancer
  • Brain metastasis
  • Craniotomy window
  • GFP
  • In vivo imaging
  • Mitosis
  • Mouse model
  • RFP
  • Subcellular dynamics

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology


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