Immunochemical detection of food-derived isothiocyanate as a lysine conjugate

Toshiyuki Nakamura; Noritoshi Kitamoto; Toshihiko Osawa; Yoji Kato

doi:10.1271/bbb.90728

Immunochemical detection of food-derived isothiocyanate as a lysine conjugate

Toshiyuki Nakamura, Noritoshi Kitamoto, Toshihiko Osawa, Yoji Kato

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

In a previous study we prepared monoclonal antibody against allyl isothiocyanate (AITC)-modified lysine (Lys), and found that AITC reacted with Lys under physiological conditions in vitro (T. Nakamura et al., Chem. Res. Toxicol., 22, 536-542 (2009)). In the present study, antibodies against benzyl isothiocyanate (ITC), 6-methylsulfinylhexyl ITC and phenethyl ITC modified protein were prepared, and the respective monoclonal antibodies, B6C9, 6MS3D10, and PE3A10 were obtained. These antibodies were applied to ITC detection in food using shredded Wasabia japonica (wasabi) and ground Carica papaya (papaya) seed by trapping ITC with biotin-labeled bovine serum albumin. ITC formation from the wasabi and papaya seed samples was confirmed using the antibodies in a dose-dependent manner. These antibodies might be applicable in identifying food-derived ITC.

Original language	English
Pages (from-to)	536-540
Number of pages	5
Journal	Bioscience, Biotechnology and Biochemistry
Volume	74
Issue number	3
DOIs	https://doi.org/10.1271/bbb.90728
Publication status	Published - 2010
Externally published	Yes

Keywords

Antibody
Enzyme-linked immunosorbent assay
Isothiocyanate
Lysine adduct

ASJC Scopus subject areas

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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title = "Immunochemical detection of food-derived isothiocyanate as a lysine conjugate",

abstract = "In a previous study we prepared monoclonal antibody against allyl isothiocyanate (AITC)-modified lysine (Lys), and found that AITC reacted with Lys under physiological conditions in vitro (T. Nakamura et al., Chem. Res. Toxicol., 22, 536-542 (2009)). In the present study, antibodies against benzyl isothiocyanate (ITC), 6-methylsulfinylhexyl ITC and phenethyl ITC modified protein were prepared, and the respective monoclonal antibodies, B6C9, 6MS3D10, and PE3A10 were obtained. These antibodies were applied to ITC detection in food using shredded Wasabia japonica (wasabi) and ground Carica papaya (papaya) seed by trapping ITC with biotin-labeled bovine serum albumin. ITC formation from the wasabi and papaya seed samples was confirmed using the antibodies in a dose-dependent manner. These antibodies might be applicable in identifying food-derived ITC.",

keywords = "Antibody, Enzyme-linked immunosorbent assay, Isothiocyanate, Lysine adduct",

author = "Toshiyuki Nakamura and Noritoshi Kitamoto and Toshihiko Osawa and Yoji Kato",

year = "2010",

doi = "10.1271/bbb.90728",

language = "English",

volume = "74",

pages = "536--540",

journal = "Bioscience, Biotechnology and Biochemistry",

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publisher = "Japan Society for Bioscience Biotechnology and Agrochemistry",

number = "3",

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TY - JOUR

T1 - Immunochemical detection of food-derived isothiocyanate as a lysine conjugate

AU - Nakamura, Toshiyuki

AU - Kitamoto, Noritoshi

AU - Osawa, Toshihiko

AU - Kato, Yoji

PY - 2010

Y1 - 2010

N2 - In a previous study we prepared monoclonal antibody against allyl isothiocyanate (AITC)-modified lysine (Lys), and found that AITC reacted with Lys under physiological conditions in vitro (T. Nakamura et al., Chem. Res. Toxicol., 22, 536-542 (2009)). In the present study, antibodies against benzyl isothiocyanate (ITC), 6-methylsulfinylhexyl ITC and phenethyl ITC modified protein were prepared, and the respective monoclonal antibodies, B6C9, 6MS3D10, and PE3A10 were obtained. These antibodies were applied to ITC detection in food using shredded Wasabia japonica (wasabi) and ground Carica papaya (papaya) seed by trapping ITC with biotin-labeled bovine serum albumin. ITC formation from the wasabi and papaya seed samples was confirmed using the antibodies in a dose-dependent manner. These antibodies might be applicable in identifying food-derived ITC.

AB - In a previous study we prepared monoclonal antibody against allyl isothiocyanate (AITC)-modified lysine (Lys), and found that AITC reacted with Lys under physiological conditions in vitro (T. Nakamura et al., Chem. Res. Toxicol., 22, 536-542 (2009)). In the present study, antibodies against benzyl isothiocyanate (ITC), 6-methylsulfinylhexyl ITC and phenethyl ITC modified protein were prepared, and the respective monoclonal antibodies, B6C9, 6MS3D10, and PE3A10 were obtained. These antibodies were applied to ITC detection in food using shredded Wasabia japonica (wasabi) and ground Carica papaya (papaya) seed by trapping ITC with biotin-labeled bovine serum albumin. ITC formation from the wasabi and papaya seed samples was confirmed using the antibodies in a dose-dependent manner. These antibodies might be applicable in identifying food-derived ITC.

KW - Antibody

KW - Enzyme-linked immunosorbent assay

KW - Isothiocyanate

KW - Lysine adduct

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U2 - 10.1271/bbb.90728

DO - 10.1271/bbb.90728

M3 - Article

C2 - 20208364

AN - SCOPUS:77950135036

SN - 0916-8451

VL - 74

SP - 536

EP - 540

JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

IS - 3

ER -

Immunochemical detection of food-derived isothiocyanate as a lysine conjugate

Abstract

Keywords

ASJC Scopus subject areas

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