Immunohistochemical expression of osterix appearing in the mouse orthodontic periodontal tension sides

Toshihisa Harada, Keisuke Nakano, Hirokazu Matsuda, Rina Muraoka, Maki Tomoda, Yukiko Yokoi, Kazuhiro Yamada, Norimasa Okafuji

Research output: Contribution to journalArticlepeer-review


Early changes of Osterix expression were examined by immunohistochemistry (IHC) and fluorescent immunohistochemistry (FIHC) in mouse periodontal ligament exposed to experimentally induced orthodontic mechanical stress. Eight-week-old ddY mice were used as experimental animals. To provide continuous orthodontic mechanical stress on the periodontal ligament, a rubber dam sheet was placed between the upper molars of the mice. At 20 min, 1 h, 3 h, 9 h and 24 h after the insertion, related parts of the animal tissues were evaluated by IHC and FIHC regarding Osterix and Runx2 expressions. In the 3-h and 9-h experimental groups, orthodontic tension sites reacted positively. The positive reactions became weak in the experimental group at 24-h. The results suggest that Osterix acts as one of the key factors of osteogenic cell differentiation, which expresses the following after the expression of Runx2, down-steam of Runx2.

Original languageEnglish
Pages (from-to)321-328
Number of pages8
JournalJournal of Hard Tissue Biology
Issue number3
Publication statusPublished - 2012
Externally publishedYes


  • Immunohistochemistry
  • Orthodontic mechanical stress
  • Osterix
  • Periodontal tissues
  • Runx2

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Biochemistry
  • Biomaterials
  • Orthopedics and Sports Medicine
  • General Dentistry
  • Cell Biology


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