Immunohistochemical Study of Collagen Type IV Alpha Chains and MMP-2, -9 at the Basement Membrane in Oral Carcinogenesis

Ryo Tamamura, Hitoshi Nagatsuka, Mehmet Gunduz, Ichiro Naitoh, Noriyuki Nagai, Lihong Ge, Yoshikazu Sado, Adusa Yabe

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


Destruction of basement membrane is an important element in invasion of cancer cells. Type IV collagen, the major component of basement membrane, has six distinct a chains. Matrix metalloproteinases (MMPs) are enzymes that resolve extracellular matrix. Oral squamous cell carcinoma is occurred after precancerous lesions, epithelial dysplasia and carcinoma in situ. We investigated localization of six a chains and MMPs in normal oral mucosal tissue, precancerous lesions, early squamous cell carcinoma immunohistochemically. In normal oral mucosal tissue and epithelial dysplasia, α1 (IV), α2 (IV), α5 (IV) and α 6 (IV) chains were detected continuously along basement membrane. In carcinoma in situ and early squamous cell carcinoma, either α5 (IV) and α6 (IV) chains or all a chains were not stained. In contrast, MMP-2 and MMP-9 that are members of type IV collagenase were stained at the parts of disappearance of a chains. This study suggested that the disappearance of a chains is an important element in carcinogenesis of oral squamous cell carcinoma.

Original languageEnglish
Pages (from-to)237-238
Number of pages2
JournalJournal of Hard Tissue Biology
Issue number2
Publication statusPublished - 2005

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Biochemistry
  • Biomaterials
  • Orthopedics and Sports Medicine
  • Dentistry(all)
  • Cell Biology


Dive into the research topics of 'Immunohistochemical Study of Collagen Type IV Alpha Chains and MMP-2, -9 at the Basement Membrane in Oral Carcinogenesis'. Together they form a unique fingerprint.

Cite this