TY - JOUR
T1 - Immunological Detection of F1-ATPase γ-Subunit in Rat Liver Peroxisomes
AU - Imanaka, Tsuneo
AU - Shiina, Yu’ichi
AU - Moriyama, Yoshinori
AU - Ohkuma, Shoji
AU - Takano, Tatsuya
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 1993/9/15
Y1 - 1993/9/15
N2 - The γ-subunit of F1-ATPase was detected in highly purified rat liver peroxisomes by immunoblot analysis using an antiserum against purified mitochondrial F1-ATPase. Other subunits of F1-ATPase such as α, β and δ were not detected in the peroxisomes. The polypeptide corresponding to the γ-subunit in peroxisomes was also recognized by affinity purified antibody against F1-ATPase γ-subunit. The amount of γ-subunit in liver homogenate increased in parallel with proliferation of peroxisomes after administration of clofibrate in rats, whereas the amounts of α, β and δ-subunits were not increased. These results suggest that the γ-subunit of F1-ATPase is a constituent of peroxisomes in rat liver.
AB - The γ-subunit of F1-ATPase was detected in highly purified rat liver peroxisomes by immunoblot analysis using an antiserum against purified mitochondrial F1-ATPase. Other subunits of F1-ATPase such as α, β and δ were not detected in the peroxisomes. The polypeptide corresponding to the γ-subunit in peroxisomes was also recognized by affinity purified antibody against F1-ATPase γ-subunit. The amount of γ-subunit in liver homogenate increased in parallel with proliferation of peroxisomes after administration of clofibrate in rats, whereas the amounts of α, β and δ-subunits were not increased. These results suggest that the γ-subunit of F1-ATPase is a constituent of peroxisomes in rat liver.
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U2 - 10.1006/bbrc.1993.2147
DO - 10.1006/bbrc.1993.2147
M3 - Article
C2 - 8373382
AN - SCOPUS:0027337945
SN - 0006-291X
VL - 195
SP - 1027
EP - 1034
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -