@article{b743592602c24fe3876228ea31964482,
title = "Improved assay system for acidic peptide: N-glycanase (aPNGase) activity in plant extracts",
abstract = "Plant acidic peptide: N-glycanase (aPNGase) release N-glycans from glycopeptides during the degradation process of glycoproteins in developing or growing plants. We have previously developed a new method to detect the aPNGase activity in crude extracts, which is prerequisite for the construction of aPNGase knockout or overexpression lines. However, this method has the disadvantage of requiring de-sialylation treatment and a lectin chromatography. In this study, therefore, we improved the simple and accurate method for detecting aPNGase activity using anion-exchange HPLC requiring neither the desialylation treatment nor the lectin affinity chromatography.",
keywords = "Acidic PNGase, Arabidopsis thaliana, Free N-Glycans, PNGase assay, Transgenic plant",
author = "Chiharu Yamamoto and Mikako Ogura and Ryota Uemura and Maeda Megumi and Hiroyuki Kajiura and Ryo Misaki and Kazuhito Fujiyama and Yoshinobu Kimura",
note = "Funding Information: This work was supported in part by grants from the Ministry of Education, Culture, Sports, Science, and Technology of Japan (Basic Research C, nos. 15K07841 and 18K05559 to M.M. and nos. 17K08197 and 20K05959 to Y.K.). We are grateful to the Department of Instrumental Analysis, Advanced Science Research Center, Okayama University, for their assistance in performing the ESI-MS analysis. Funding Information: This work was supported in part by grants from the Ministry of Education , Culture, Sports, Science, and Technology of Japan (Basic Research C, nos. 15K07841 and 18K05559 to M.M. and nos. 17K08197 and 20K05959 to Y.K.). We are grateful to the Department of Instrumental Analysis, Advanced Science Research Center, Okayama University, for their assistance in performing the ESI-MS analysis. Publisher Copyright: {\textcopyright} 2021 Elsevier Inc.",
year = "2021",
month = dec,
day = "1",
doi = "10.1016/j.ab.2021.114367",
language = "English",
volume = "634",
journal = "Analytical Biochemistry",
issn = "0003-2697",
publisher = "Academic Press Inc.",
}