Improved bioassay method for Spodoptera litura chitinase inhibitors using a colloidal chitin powder with a uniform particle size as substrate

T. Nitoda; H. Kurumatani; H. Kanzaki; K. Kawazu

doi:10.1002/(sici)1096-9063(199905)55:5<563::aid-ps972>3.0.co;2-%23

Improved bioassay method for Spodoptera litura chitinase inhibitors using a colloidal chitin powder with a uniform particle size as substrate

T. Nitoda, H. Kurumatani, H. Kanzaki, K. Kawazu

School of Agriculture

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

A previously reported bioassay method for Spodoptera litura chitinase inhibitors has been improved by use of colloidal chitin powder with a uniform particle size. This improvement made the assay four times more sensitive. Detection of three active supernatants by screening of supernatants and cell extracts from 135 fermentation broths has proved the efficiency of this improved method.

Original language	English
Pages (from-to)	563-565
Number of pages	3
Journal	Pesticide Science
Volume	55
Issue number	5
DOIs	https://doi.org/10.1002/(sici)1096-9063(199905)55:5<563::aid-ps972>3.0.co;2-%23
Publication status	Published - Jan 1 1999

Keywords

Chitinase inhibitory assay
Colloidal chitin
Spodoptera litura

ASJC Scopus subject areas

Applied Microbiology and Biotechnology

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10.1002/(sici)1096-9063(199905)55:5<563::aid-ps972>3.0.co;2-%23

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AU - Kurumatani, H.

AU - Kanzaki, H.

AU - Kawazu, K.

PY - 1999/1/1

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N2 - A previously reported bioassay method for Spodoptera litura chitinase inhibitors has been improved by use of colloidal chitin powder with a uniform particle size. This improvement made the assay four times more sensitive. Detection of three active supernatants by screening of supernatants and cell extracts from 135 fermentation broths has proved the efficiency of this improved method.

AB - A previously reported bioassay method for Spodoptera litura chitinase inhibitors has been improved by use of colloidal chitin powder with a uniform particle size. This improvement made the assay four times more sensitive. Detection of three active supernatants by screening of supernatants and cell extracts from 135 fermentation broths has proved the efficiency of this improved method.

KW - Chitinase inhibitory assay

KW - Colloidal chitin

KW - Spodoptera litura

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Improved bioassay method for Spodoptera litura chitinase inhibitors using a colloidal chitin powder with a uniform particle size as substrate

Abstract

Keywords

ASJC Scopus subject areas

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