In photoinhibited photosystem II particles pheophytin photoreduction remains unimpaired

Suleiman I. Allakhverdiev; Eva Šetlíková; Vyacheslav V. Klimov; Ivan Šetlík

doi:10.1016/0014-5793(87)80576-8

In photoinhibited photosystem II particles pheophytin photoreduction remains unimpaired

Suleiman I. Allakhverdiev, Eva Šetlíková, Vyacheslav V. Klimov, Ivan Šetlík

Research output: Contribution to journal › Article › peer-review

72 Citations (Scopus)

Abstract

Oxygen-evolving photosystem II particles (DT 20) isolated from pea chloroplasts by digitonin-Triton X-100 fractionation were photoinhibited with 150 W·m^-2 white light, at 20°C under three conditions: aerobic, anaerobic and strongly reducing (E_h poised to approx. -250 mV with dithionite). Hill reaction rate (H₂O → BQ) and variable fluorescence (F_v) declined in parallel in all three cases with shortening half times: 30, 10 and 2.5 min, respectively. Light-induced absorbance changes at 685 nm characteristic of reversible photoaccumulation of reduced pheophytin (E_h ≈ -250 mV) remained essentially unchanged. We conclude that the three types of photoinhibitory treatment do not impair the separation of charges between chlorophyll P-680 and pheophytin in the photosystem II reaction center.

Original language	English
Pages (from-to)	186-190
Number of pages	5
Journal	FEBS Letters
Volume	226
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(87)80576-8
Publication status	Published - Dec 21 1987

Keywords

(Pea chloroplasts)
Charge separation
Oxygen-evolving PS II particle
Pheophytin
Photoinhibition

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

Access to Document

10.1016/0014-5793(87)80576-8

Cite this

@article{0a5e72ee1dfd4aa9a960030588d86a04,

title = "In photoinhibited photosystem II particles pheophytin photoreduction remains unimpaired",

abstract = "Oxygen-evolving photosystem II particles (DT 20) isolated from pea chloroplasts by digitonin-Triton X-100 fractionation were photoinhibited with 150 W·m-2 white light, at 20°C under three conditions: aerobic, anaerobic and strongly reducing (Eh poised to approx. -250 mV with dithionite). Hill reaction rate (H2O → BQ) and variable fluorescence (Fv) declined in parallel in all three cases with shortening half times: 30, 10 and 2.5 min, respectively. Light-induced absorbance changes at 685 nm characteristic of reversible photoaccumulation of reduced pheophytin (Eh ≈ -250 mV) remained essentially unchanged. We conclude that the three types of photoinhibitory treatment do not impair the separation of charges between chlorophyll P-680 and pheophytin in the photosystem II reaction center.",

keywords = "(Pea chloroplasts), Charge separation, Oxygen-evolving PS II particle, Pheophytin, Photoinhibition",

author = "Allakhverdiev, {Suleiman I.} and Eva {\v S}etl{\'i}kov{\'a} and Klimov, {Vyacheslav V.} and Ivan {\v S}etl{\'i}k",

year = "1987",

month = dec,

day = "21",

doi = "10.1016/0014-5793(87)80576-8",

language = "English",

volume = "226",

pages = "186--190",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Elsevier",

number = "1",

}

TY - JOUR

T1 - In photoinhibited photosystem II particles pheophytin photoreduction remains unimpaired

AU - Allakhverdiev, Suleiman I.

AU - Šetlíková, Eva

AU - Klimov, Vyacheslav V.

AU - Šetlík, Ivan

PY - 1987/12/21

Y1 - 1987/12/21

N2 - Oxygen-evolving photosystem II particles (DT 20) isolated from pea chloroplasts by digitonin-Triton X-100 fractionation were photoinhibited with 150 W·m-2 white light, at 20°C under three conditions: aerobic, anaerobic and strongly reducing (Eh poised to approx. -250 mV with dithionite). Hill reaction rate (H2O → BQ) and variable fluorescence (Fv) declined in parallel in all three cases with shortening half times: 30, 10 and 2.5 min, respectively. Light-induced absorbance changes at 685 nm characteristic of reversible photoaccumulation of reduced pheophytin (Eh ≈ -250 mV) remained essentially unchanged. We conclude that the three types of photoinhibitory treatment do not impair the separation of charges between chlorophyll P-680 and pheophytin in the photosystem II reaction center.

AB - Oxygen-evolving photosystem II particles (DT 20) isolated from pea chloroplasts by digitonin-Triton X-100 fractionation were photoinhibited with 150 W·m-2 white light, at 20°C under three conditions: aerobic, anaerobic and strongly reducing (Eh poised to approx. -250 mV with dithionite). Hill reaction rate (H2O → BQ) and variable fluorescence (Fv) declined in parallel in all three cases with shortening half times: 30, 10 and 2.5 min, respectively. Light-induced absorbance changes at 685 nm characteristic of reversible photoaccumulation of reduced pheophytin (Eh ≈ -250 mV) remained essentially unchanged. We conclude that the three types of photoinhibitory treatment do not impair the separation of charges between chlorophyll P-680 and pheophytin in the photosystem II reaction center.

KW - (Pea chloroplasts)

KW - Charge separation

KW - Oxygen-evolving PS II particle

KW - Pheophytin

KW - Photoinhibition

UR - http://www.scopus.com/inward/record.url?scp=0000655682&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0000655682&partnerID=8YFLogxK

U2 - 10.1016/0014-5793(87)80576-8

DO - 10.1016/0014-5793(87)80576-8

M3 - Article

C2 - 22872911

AN - SCOPUS:0000655682

SN - 0014-5793

VL - 226

SP - 186

EP - 190

JO - FEBS Letters

JF - FEBS Letters

IS - 1

ER -

In photoinhibited photosystem II particles pheophytin photoreduction remains unimpaired

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this