In photoinhibited photosystem II particles pheophytin photoreduction remains unimpaired

Suleiman I. Allakhverdiev, Eva Šetlíková, Vyacheslav V. Klimov, Ivan Šetlík

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    72 Citations (Scopus)


    Oxygen-evolving photosystem II particles (DT 20) isolated from pea chloroplasts by digitonin-Triton X-100 fractionation were photoinhibited with 150 W·m-2 white light, at 20°C under three conditions: aerobic, anaerobic and strongly reducing (Eh poised to approx. -250 mV with dithionite). Hill reaction rate (H2O → BQ) and variable fluorescence (Fv) declined in parallel in all three cases with shortening half times: 30, 10 and 2.5 min, respectively. Light-induced absorbance changes at 685 nm characteristic of reversible photoaccumulation of reduced pheophytin (Eh ≈ -250 mV) remained essentially unchanged. We conclude that the three types of photoinhibitory treatment do not impair the separation of charges between chlorophyll P-680 and pheophytin in the photosystem II reaction center.

    Original languageEnglish
    Pages (from-to)186-190
    Number of pages5
    JournalFEBS Letters
    Issue number1
    Publication statusPublished - Dec 21 1987


    • (Pea chloroplasts)
    • Charge separation
    • Oxygen-evolving PS II particle
    • Pheophytin
    • Photoinhibition

    ASJC Scopus subject areas

    • Biophysics
    • Structural Biology
    • Biochemistry
    • Molecular Biology
    • Genetics
    • Cell Biology


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