In situ analysis of the removal behavior of protein adsorbed on a titanium surface by H₂O₂-electrolysis treatment

The kinetics of the removal of β-lactoglobulin (β-Lg) adsorbed to a titanium surface using a method involving the electrolysis of hydrogen peroxide (H₂O₂-electrolysis treatment) was examined. The rate of decrease in the thickness of the layer β-Lg adsorbed to the titanium surface during the H₂Q₂-electrolysis treatment was measured in situ by ellipsometry. The rate for this process followed first-order reaction kinetics. Relationships between the rate constant for removal and such factors as the H₂O₂ concentration, the potential applied to the titanium surface, the supporting electrolyte concentration, and temperature were experimentally studied. The rate of removal of the adsorbed β-Lg from a stainless steel surface was found to be much slower than that from a titanium surface, indicating that the type of metal also affects the removal characteristics during the H₂O₂-electrolysis treatment.