TY - JOUR
T1 - In situ localization of rat testis-specific calcineurin B subunit isoform β1 in the developing rat testis
AU - Miyamoto, Kazuhiro
AU - Matsui, Hideki
AU - Tomizawa, Kazuhito
AU - Kuwata, Yoshihiro
AU - Itano, Toshifumi
AU - Tokuda, Masaaki
AU - Hatase, Osamu
PY - 1994/9/15
Y1 - 1994/9/15
N2 - In situ localization and developmental changes in expression of testis-specific calcineurin B subunit isoform β1 was examined in rat testis. Two different sizes of mRNA signal, 4.0 kb and 0.9 kb, were detected by Northern blot hybridization. Both signals were expressed synchronously with the start of meiosis at 3 weeks after birth, and increased depending on the maturation of spermatogenesis. In situ hybridization using non-radioactive riboprobes showed that the β1 mRNA was specifically localized to spermatocytes where meiosis occurs but none or very little was observed in spermatogonia, spermatids, Sertoli or Leydig cells.
AB - In situ localization and developmental changes in expression of testis-specific calcineurin B subunit isoform β1 was examined in rat testis. Two different sizes of mRNA signal, 4.0 kb and 0.9 kb, were detected by Northern blot hybridization. Both signals were expressed synchronously with the start of meiosis at 3 weeks after birth, and increased depending on the maturation of spermatogenesis. In situ hybridization using non-radioactive riboprobes showed that the β1 mRNA was specifically localized to spermatocytes where meiosis occurs but none or very little was observed in spermatogonia, spermatids, Sertoli or Leydig cells.
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U2 - 10.1006/bbrc.1994.2320
DO - 10.1006/bbrc.1994.2320
M3 - Article
C2 - 8093044
AN - SCOPUS:0028000350
SN - 0006-291X
VL - 203
SP - 1275
EP - 1283
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -