TY - JOUR
T1 - In vitro cytocompatibility of MG63 cells on chitosan-organosiloxane hybrid membranes
AU - Shirosaki, Yuki
AU - Tsuru, Kanji
AU - Hayakawa, Satoshi
AU - Osaka, Akiyoshi
AU - Lopes, Maria Ascensão
AU - Santos, José Domingos
AU - Fernandes, Maria Helena
N1 - Funding Information:
The authors wish to acknowledge the financial support of FCT-Fundação para a Ciéncia e Tecnologia through the project entitled “Interactive calcium–phosphate based materials prepared by post-hybridisation and in situ hybridisation”, ref: PCTI/1999/CTM/35516.
PY - 2005/2
Y1 - 2005/2
N2 - Chitosan-silicate hybrids were synthesized using γ- glycidoxypropyltrimethoxysilane (GPSM) as the agent for cross-linking the chitosan chains. CaCl2 was introduced in the hybrids in expectation that it would improve cell adhesion and differentiation of the hybrid surfaces. Fourier-transform infrared (FT-IR) spectroscopy and 29Si CP-MAS NMR spectroscopy were used to analyze the structures of the hybrids. Cytocompatibility of the hybrids was investigated in terms of proliferation of an osteoblastic cell line, MG63. The adhesion and proliferation of the osteoblastic cells cultured on the surface of a chitosan-GPSM hybrid without calcium were similar to those on a control culture plate, and were better than those on a chitosan membrane. The ALP activity of the cells cultured on this hybrid was higher than that on the chitosan membrane. Contrary to expectations, the incorporation of calcium ions into the hybrids did not improve cell attachment and proliferation on their surfaces.
AB - Chitosan-silicate hybrids were synthesized using γ- glycidoxypropyltrimethoxysilane (GPSM) as the agent for cross-linking the chitosan chains. CaCl2 was introduced in the hybrids in expectation that it would improve cell adhesion and differentiation of the hybrid surfaces. Fourier-transform infrared (FT-IR) spectroscopy and 29Si CP-MAS NMR spectroscopy were used to analyze the structures of the hybrids. Cytocompatibility of the hybrids was investigated in terms of proliferation of an osteoblastic cell line, MG63. The adhesion and proliferation of the osteoblastic cells cultured on the surface of a chitosan-GPSM hybrid without calcium were similar to those on a control culture plate, and were better than those on a chitosan membrane. The ALP activity of the cells cultured on this hybrid was higher than that on the chitosan membrane. Contrary to expectations, the incorporation of calcium ions into the hybrids did not improve cell attachment and proliferation on their surfaces.
KW - Chitosan
KW - Cytocompatibility
KW - MG63 osteoblastic cells
KW - Organic-inorganic hybrid
KW - γ-Glycidoxypropyltrimethoxysilane (GPSM)
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U2 - 10.1016/j.biomaterials.2004.02.056
DO - 10.1016/j.biomaterials.2004.02.056
M3 - Article
C2 - 15276356
AN - SCOPUS:3242791810
SN - 0142-9612
VL - 26
SP - 485
EP - 493
JO - Biomaterials
JF - Biomaterials
IS - 5
ER -